Stratification of anaerobic methanotrophs in cold-seep sediments

doi:10.15468/ipnigo

Stratification of anaerobic methanotrophs in cold-seep sediments

Citation

MGnify (2019). Stratification of anaerobic methanotrophs in cold-seep sediments. Sampling event dataset https://doi.org/10.15468/ipnigo accessed via GBIF.org on 2022-01-28.

Description

Methane seepages typically harbor communities of anaerobic methane oxidizers (ANME), however knowledge about fine-scale vertical variation of ANME in response to geochemical gradients is limited. Here we investigate microbial communities in sediments at two different locations at thge Nyegga field; (1) a push-core below a white microbial mat in the G11 pockmark, and (2) a Gravity-core at CNO3. Both experiments were analysed using 16S rRNA gene tag pyrosequencing and real time quantitative PCR.

Sampling Description

Sampling

Methane seepages typically harbor communities of anaerobic methane oxidizers (ANME), however knowledge about fine-scale vertical variation of ANME in response to geochemical gradients is limited. Here we investigate microbial communities in sediments at two different locations at thge Nyegga field; (1) a push-core below a white microbial mat in the G11 pockmark, and (2) a Gravity-core at CNO3. Both experiments were analysed using 16S rRNA gene tag pyrosequencing and real time quantitative PCR.

Method steps

Pipeline used: https://www.ebi.ac.uk/metagenomics/pipelines/4.1

Taxonomic Coverages

Geographic Coverages

Bibliographic Citations

Roalkvam I, Jørgensen SL, Chen Y, Stokke R, Dahle H, Hocking WP, Lanzén A, Haflidason H, Steen IH. 2011. New insight into stratification of anaerobic methanotrophs in cold seep sediments. null vol. 78 - DOI:10.1111/j.1574-6941.2011.01153.x

Contacts

originator
University of Bergen

metadata author
University of Bergen

administrative point of contact
University of Bergen