BCCM/ULC Cyanobacteria culture collection at the University of Liège (ISO9001 certified)

Annick Wilmotte; Kim Beets

doi:10.15468/jrcrb7

BCCM/ULC Cyanobacteria culture collection at the University of Liège (ISO9001 certified)

Citation

Wilmotte A, Beets K (2022): BCCM/ULC Cyanobacteria culture collection at the University of Liège (ISO9001 certified). v1.38. SCAR - Microbial Antarctic Resource System. Dataset/Occurrence. https://ipt.biodiversity.aq/resource?r=bccm_ulc_culture_collection_uliege&v=1.38 https://doi.org/10.15468/jrcrb7 accessed via GBIF.org on 2023-09-25.

Description

BCCM/ULC is a small and dedicated public culture collection, currently containing one of the largest collections of documented (sub)polar cyanobacteria worldwide.

The BCCM/ULC collection is hosted by the Centre for Protein Engineering (the Unit) of the University of Liège. The host Unit is very active in research projects concerning the cyanobacterial diversity and biogeography, with a focus on polar biotopes. The approach used is polyphasic, including the isolation of strains and culture-independent methods (DGGE, clone libraries, pyrosequencing based on the ribosomal operon sequences). The participation to field expeditions in the Antarctic and Arctic has enabled to collect samples in many locations. Moreover, taxonomic research is carried out by the host Unit to improve the classification of the cyanobacterial phylum. It is based on a polyphasic approach combining the morphological and molecular characterizations of the strains.

Purpose

The data set was created to document the origin of the strains in the BCCM/ULC collection

Sampling Description

Study Extent

The BCCM/ULC cyanobacteria culture collection accepts the deposits (public and safe) of all kinds of cyanobacteria, if they correspond to its cultivation capacities and are not duplicates of already existing strains.

Sampling

The host laboratory has one of the largest research collections of documented (sub)polar cyanobacteria worldwide, with more than 100 strains characterized by phenotypic (morphology based on microscopic observations) and genotypic (16S rRNA and ITS sequences) analyses. The morphological identification shows that the strains belong to the orders Synechococcales, Oscillatoriales, Pleurocapsales, Chroococcidiopsidales and Nostocales. The strains are unicyanobacterial, but not axenic. They are available as living cultures, and the majority is also cryo-preserved (-70°C). BCCM/ULC is progressively incorporating the most interesting strains from the research collection of the host Unit into the public collection. In addition, ongoing research and public deposits from other (more temperate) geographical areas enrich progressively the variety of proposed strains. The public collection now includes the type strain of the recently described species Plectolyngbya hodgsonii (Taton et al., 2011, Polar Biology, 34: 181-191).

Quality Control

The services of deposits and public distribution are certified by an ISO9001 certificate, since 2012.

Method steps

Upon deposit, the depositors must fill the Accession form that includes the information on the origin and characteristics of the sampling site and of the srain isolated from the sampling site (http://bccm.belspo.be/docs/deposit-forms). These elements are used for the metadata.

Taxonomic Coverages

The BCCM/ULC culture collection exclusively contains Cyanobacterial strains

Cyanobacteria
common name: Cyanobacteria rank: phylum

Geographic Coverages

Global coverage, mainly Antarctica, Europe and the Americas

Bibliographic Citations

Taton, A., Grubisic, S., Ertz, D., Hodgson, D., Piccardi, R., Biondi, N., Tredici, M., Mainini, M., Losi, D., Marinelli, F., & Wilmotte, A. (2006). Polyphasic study of Antarctic cyanobacterial strains. Journal of Phycology, 42(6), 1257-1270. http://hdl.handle.net/2268/19904 -
Biondi, N., Tredici, M., Taton, A., Wilmotte, A., Hodgson, D., Losi, D., & Marinelli, F. (2008). Cyanobacteria from benthic mats of Antarctic lakes as a source of new bioactivities. Journal of Applied Microbiology, 105(1), 105-115. http://hdl.handle.net/2268/17380 -
A. Dumas, G. Laliberté, P. Lessard, J. de la Noüe, Biotreatment of fish farm effluents using the cyanobacterium Phormidium bohneri, Aquacultural Engineering, Volume 17, Issue 1, February 1998, Pages 57-68, ISSN 0144-8609, 10.1016/S0144-8609(97)01013-3. -
P. Chevalier, D. Proulx, P. Lessard, W.F. Vincent and J. de la Noüe. (2000) Nitrogen and phosphorus removal by high latitude mat-forming cyanobacteria for potential use in tertiary wastewater treatment Journal of Applied Phycology Volume 12, Number 2, 105-112, DOI: 10.1023/A:1008168128654 - Talbot P. & De la Noue. J (1993) Tertiary treatment of wastewater with Phormidium bohneri (Schmidle) under various light and temperature conditions. Water Research, vol. 27, pp. 153-159 -
Nadeau T-L, Milbrandt EC & RC Castenholz (2001). Evolutionary relationships of cultivated Oscillatorians (Cyanobacteria). J. Phycol. 37: 650-654. -
Strunecki, O, Komarek, J, Johansen J, Lukesova A & Elster J. 2013. Molecular and morphological criteria for revision of the genus Microcoleus (Oscillatoriales, Cyanobacteria). J. Phycol. 49, 1167-1180. -
Jancusova, M., Kovacik, L., Pereira, A. B., Dusinsky, R., & Wilmotte, A. (2016). Polyphasic characterization of 10 selected ecologically relevant filamentous cyanobacterial strains from the South Shetland Islands, Maritime Antarctica. FEMS Microbiology Ecology, 92 (7), 100 -
Sciuto K., Moschin E., Moro I. (2017) Cryptic cyanobacterial taxa from the Giant Cave (Triste Italy) Hydrobiologia; Katia Sciuto, Emanuela Moschin, and Isabella Moro (2017) Cryptic Cyanobacterial Diversity in the Giant Cave (Trieste, Italy): The New Genus Timaviella (Leptolyngbyaceae) Cryptogamie, Algologie 38 (4), 285-323 -
Kopf M, Möke F, Bauwe H, Hess WR, Hagemann M. (2015). Expression profiling of the bloom-forming cyanobacterium Nodularia CCY9414 under light and oxidative stress conditions. ISME Journal 9: 2139–2152. -

Contacts

Annick Wilmotte
originator
BCCM/ULC culture collection at the University of Liège
allée du 6 aout, 11
Liège
4000
Wallonia
BE
Telephone: +32-(0)4-366 33 87
email: BCCM.ULC@ulg.ac.be
homepage: http://bccm.belspo.be/about-us/bccm-ulc

Kim Beets
originator
position: Technical assistant
BCCM/ULC culture collection at the University of Liège
Institute of Chemistry B6 Sart Tilman
Liège
4000
Wallonia
BE
Telephone: +32-(0)4-366 33 87
homepage: http://bccm.belspo.be/about-us/bccm-ulc

Maxime Sweetlove
metadata author
position: Research assistent
Royal Belgian Institute for Natural Sciences
Rue Vautier 29
Brussels
1000
Brussels
BE
email: msweetlove@naturalsciences.be

Annick Wilmotte
metadata author
position: Promotor
BCCM/ULC culture collection at the University of Liège
Allée du 6 aout, 11
Liège
4000
Wallonia
BE
Telephone: +32-(0)4-366 33 87
email: BCCM.ULC@ulg.ac.be

Paulette Charlier
administrative point of contact
position: Director
InBios-Centre for Protein Engineering
Allée du 6 aout, 11
Liège
4000
Wallonia
BE
email: paulette.charlier@uliege.be

Annick Wilmotte
administrative point of contact
position: Promotor
BCCM/ULC culture collection at University of Liège
Allée du 6 aout, 11
Liège
4000
Wallonia
BE
Telephone: +32-(0)4-366 33 87
email: BCCM.ULC@ulg.ac.be
homepage: http://bccm.belspo.be/about-us/bccm-ulc