Chironomid Specimen records in the Chironomid DNA Barcode Database.

Study Extent

Chironomid species were captured in Japan and Tuvalu from 1989 to 2015.

Sampling

Specimens were collected in three major ways. (1) Larvae and pupae in bottom sediments were captured with a Ekman bottom grab sampler or a dip net in lentic waters and with a Surber sampler or a D-frame net in lotic waters. Aquatic weeds were also hand-collected for periphytic species. (2) A part of chironomids captured underwater was reared to adults in the laboratory. They were kept in water aerated with a pump in tanks. (3) Midges were collected with an emergence trap or a sweeping net on and around waters where they emerged.

Quality Control

Scientific names were validated against the Catalogue of the Insects of Japan Vol.8 (Yamamoto and Yamamoto 2014) and the GBIF Backbone Taxonomy.

Method steps

1. Morphological identification was used to determine the species names of specimens, along with DNA barcoding and ecological observations for a limited number of species. Geographic coordinates and uncertainties were measured using gazetteers such as Google Maps following procedures described in Chapman and Wieczorek (2006). Chironomid specimen records were extracted from the Chironomid DNA Barcode Database and organized into species occurrence data using a MySQL database.

1. Ablabesmyia
   rank: genus
2. Ainuyusurika
   rank: genus
3. Benthalia
   rank: genus
4. Biwatendipes
   rank: genus
5. Brillia
   rank: genus
6. Bryophaenocladius
   rank: genus
7. Cardiocladius
   rank: genus
8. Chironomus
   rank: genus
9. Cladopelma
   rank: genus
10. Cladotanytarsus
    rank: genus
11. Compterosmittia
    rank: genus
12. Conchapelopia
    rank: genus
13. Cricotopus
    rank: genus
14. Cryptochironomus
    rank: genus
15. Demicryptochironomus
    rank: genus
16. Diamesa
    rank: genus
17. Dicrotendipes
    rank: genus
18. Endochironomus
    rank: genus
19. Eukiefferiella
    rank: genus
20. Euryhapsis
    rank: genus
21. Glyptotendipes
    rank: genus
22. Harnischia
    rank: genus
23. Heleniella
    rank: genus
24. Kiefferulus
    rank: genus
25. Limnophyes
    rank: genus
26. Lipiniella
    rank: genus
27. Metriocnemus
    rank: genus
28. Microchironomus
    rank: genus
29. Micropsectra
    rank: genus
30. Microtendipes
    rank: genus
31. Nanocladius
    rank: genus
32. Neozavrelia
    rank: genus
33. Nilodorum
    rank: genus
34. Nilothauma
    rank: genus
35. Orthocladius
    rank: genus
36. Parachironomus
    rank: genus
37. Paracladopelma
    rank: genus
38. Parakiefferiella
    rank: genus
39. Parametriocnemus
    rank: genus
40. Paratanytarsus
    rank: genus
41. Paratendipes
    rank: genus
42. Polypedilum
    rank: genus
43. Potthastia
    rank: genus
44. Prodiamesa
    rank: genus
45. Propsilocerus
    rank: genus
46. Psectrocladius
    rank: genus
47. Psectrotanypus
    rank: genus
48. Pseudorthocladius
    rank: genus
49. Pseudosmittia
    rank: genus
50. Rheocricotopus
    rank: genus
51. Rheopelopia
    rank: genus
52. Rheotanytarsus
    rank: genus
53. Smittia
    rank: genus
54. Stempellinella
    rank: genus
55. Stictochironomus
    rank: genus
56. Synorthocladius
    rank: genus
57. Tanypus
    rank: genus
58. Tanytarsus
    rank: genus
59. Telmatogeton
    rank: genus
60. Thienemanniella
    rank: genus
61. Thienemannimyia
    rank: genus
62. Trissopelopia
    rank: genus
63. Tvetenia
    rank: genus
64. Virgatanytarsus
    rank: genus
65. Yaetanytarsus
    rank: genus

Japan and Tuvalu