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HBIs and sterols in zooplankton Nansen Legacy Q4

Citation

Kohlbach D, Wold A, Keck Al-Habahbeh A, Schmidt K, Smik L, Belt S T, Hop H, Assmy P (2022). HBIs and sterols in zooplankton Nansen Legacy Q4. The Nansen Legacy Project. Sampling event dataset https://doi.org/10.21334/npolar.2022.37159527 accessed via GBIF.org on 2025-06-25.

Description

HBIs and sterols in pelagic zooplankton collected during Nansen Legacy seasonal cruise Q4 (28 November - 17 December 2019)

Sampling Description

Study Extent

Nansen Legacy main transect along 34°E in the Northern Barents Sea and adjacent Arctic Basin from 76 to 82°N

Sampling

Samples of 5 zooplankton taxa including copepods and amphipods were collected at six stations (P1, P4, P5, P6, P7, NLEG3), using MIK nets (1200 μm with 500 μm cod end), Macroplankton trawl (multiple mesh sizes along the net, tapering to 8 mm at its end) and Bongo nets (64 and 180 μm). Samples were sorted into species and stage/size groups onboard the ship and immediately frozen at -80 ºC.

Method steps

  1. HBIs and sterols were analysed at the University of Plymouth, UK. All samples were freeze-dried (-45 °C, 0.1 mbar, 24-48 h). Total lipids were extracted with chloroform/methanol (2:1, v/v) and cleaned with potassium chloride (0.88 %). Thereafter, samples were saponified with 20 % potassium hydroxide in water/methanol (1:9, v/v) at 70 °C for 1 h. Non-saponifiable lipids were extracted with hexane and purified by open column chromatography filled with SiO2. HBIs were eluted using hexane. The partially purified non-polar lipids containing HBIs were analysed by gas chromatography-mass spectrometry (GC-MS). Quantification of HBIs was achieved by integrating individual ion responses in single-ion monitoring mode, and normalising these to the corresponding peak area of the internal standard and an instrumental response factor obtained from purified standards (Belt et al., 2012). Sterols were eluted from the same silica column using hexane:methylacetate (4:1,v/v). Sterol fractions were derivatised using N,O-bis(trimethylsilyl)trifluoroacetamide (70 °C, 1 h) and analysed GC-MS. Individual sterols were identified by comparison of the mass spectra of their trimethylsilyl-ethers with published data (Belt et al., 2018). Belt, S.T., Brown, T.A., Sanz, P.C., and Rodriguez, A.N. (2012). Structural confirmation of the sea ice biomarker IP25 found in Arctic marine sediments. Environ. Chem. Lett. 10(2), 189-192 Belt, S.T., Brown, T.A., Smik, L., Assmy, P., and Mundy, C.J. (2018). Sterol identification in floating Arctic sea ice algal aggregates and the Antarctic sea ice diatom Berkeleya adeliensis. Org. Geochem. 118, 1-3

Taxonomic Coverages

Copepods, amphipods
  1. Calanus glacialis
  2. Calanus hyperboreus
  3. Calanus finmarchicus
  4. Themisto abyssorum
  5. Themisto libellula

Geographic Coverages

Nansen Legacy main transect along 34°E in the Northern Barents Sea and adjacent Arctic Basin from 76 to 82°N

Bibliographic Citations

  1. Kohlbach D, Schmidt K, Hop H, Wold A, Al-Habahbeh AK, Belt ST, Woll M, Graeve M, Smik L, Atkinson A and Assmy P (2021) Winter Carnivory and Diapause Counteract the Reliance on Ice Algae by Barents Sea Zooplankton. Front. Mar. Sci. 8:640050 - doi: 10.3389/fmars.2021.640050

Contacts

Doreen Kohlbach
originator
position: Postdoctoral Researcher
Norwegian Polar Institute
Tromsø
NO
email: doreen.kohlbach@npolar.no
Anette Wold
originator
position: Engineer
Norwegian Polar Institute
Tromsø
NO
email: anette.wold@npolar.no
Amalia Keck Al-Habahbeh
originator
position: PhD student
UiT The Arctic University of Norway
Tromsø
NO
email: amalia.keck@uit.no
Katrin Schmidt
originator
position: Researcher
University of Plymouth
Plymouth
GB
email: katrin.schmidt@plymouth.ac.uk
Lukas Smik
originator
position: Postdoctoral Researcher
University of Plymouth
Plymouth
GB
email: lukas.smik@plymouth.ac.uk
Simon T. Belt
originator
position: Researcher
University of Plymouth
Plymouth
GB
email: s.belt@plymouth.ac.uk
Haakon Hop
originator
position: Researcher
Norwegian Polar Institute
Tromsø
NO
email: haakon.hop@npolar.no
Philipp Assmy
originator
position: Researcher
Norwegian Polar Institute
Tromsø
NO
email: philipp.assmy@npolar.no
Doreen Kohlbach
metadata author
position: Postdoctoral Researcher
Norwegian Polar Institute
Tromsø
NO
email: doreen.kohlbach@npolar.no
Doreen Kohlbach
author
position: Postdoctoral Researcher
Norwegian Polar Institute
Tromsø
NO
email: doreen.kohlbach@npolar.no
Anette Wold
author
position: Engineer
Norwegian Polar Institute
Tromsø
NO
email: anette.wold@npolar.no
Amalia Keck Al-Habahbeh
author
position: PhD student
UiT The Arctic University of Norway
Tromsø
NO
email: amalia.keck@uit.no
Katrin Schmidt
author
position: Researcher
University of Plymouth
Plymouth
GB
email: katrin.schmidt@plymouth.ac.uk
Lukas Smik
author
position: Postdoctoral Researcher
University of Plymouth
Plymouth
GB
email: lukas.smik@plymouth.ac.uk
Simon T. Belt
author
position: Researcher
University of Plymouth
Plymouth
GB
email: s.belt@plymouth.ac.uk
Haakon Hop
author
position: Researcher
Norwegian Polar Institute
Tromsø
NO
email: haakon.hop@npolar.no
Philipp Assmy
author
position: Researcher
Norwegian Polar Institute
Tromsø
NO
email: philipp.assmy@npolar.no
Doreen Kohlbach
administrative point of contact
position: Postdoctoral Researcher
Norwegian Polar Institute
Tromsø
NO
email: doreen.kohlbach@npolar.no
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