FBIP: Genetic diversity of olive insect pests and their natural enemies in the Western Cape

Barbara van Asch

doi:10.15468/xmksze

FBIP: Genetic diversity of olive insect pests and their natural enemies in the Western Cape

Citation

van Asch B (2019). FBIP: Genetic diversity of olive insect pests and their natural enemies in the Western Cape. South African National Biodiversity Institute. Occurrence dataset https://doi.org/10.15468/xmksze accessed via GBIF.org on 2020-01-23.

Description

Assess the occurrence and infestation rate of olive flies, olive beetles, olive lace bug and their parasitoids in wild and domestic olives in the Western Cape and to generate primary DNA sequence data. The data will include estimates of genetic diversity in olive flies, novel complete mitochondrial genomes and sequences for barcoding in six species.

Sampling Description

Study Extent

Western Cape, Stellenbosch

Sampling

Whole animals where collected

Method steps

We propose to survey the presence, estimate infestation rate in wild and domestic olives and assess the genetic diversity of B. oleae and B. bigutulla, Argopistes spp. (A. sexvitattus, A. capensis and A. oleae) and P. australis (olive flies, olive beetles and olive lace bug, respectively) in the Western Cape of South Africa. We will generate primary sequence data on complete mitochondrial genomes for one individual of each species, and cytochrome c oxidase I (COI, the reference barcoding DNA region) for 50 individuals of each species. Complete mitogenomes will be used to reconstruct phylogenetic trees of Bactrocera spp., the Galerucinae sub-family for Argopistes spp. and the Tingidae family for Plerochila australis. Novel COI data will be generated and deposited in BOLD for barcoding purposes, accompanied by full taxonomic records. Complete mitogenomes, COI and mtDNA polymorphic regions will be used to assess intra-specific diversity in olive flies.

Taxonomic Coverages

Olive Insects

Insects
rank: class

Geographic Coverages

Western Cape, Stellenbosch

Bibliographic Citations

Contacts

Barbara van Asch
originator
position: Senior Lecturer
Stellenbosch University
Stellenbosch University, Department of Genetics, JC Smuts Building Room 224
Stellenbosch University
7601
Western Cape
ZA
Telephone: 0218085888
email: bva@sun.ac.za
homepage: http://www.sun.ac.za/english/faculty/agri/genetics/staff-students/academic-staff

Barbara van Asch
metadata author
position: Senior Lecturer
Stellenbosch University
Stellenbosch University, Department of Genetics, JC Smuts Building Room 224
Stellenbosch University
7601
Western Cape
ZA
Telephone: 0218085888
email: bva@sun.ac.za
homepage: http://www.sun.ac.za/english/faculty/agri/genetics/staff-students/academic-staff

Mahlatse Kgatla
content provider
position: FBIP Data Specialist
SANBI
2 Cussonia Avenue, Brummeria
Pretoria
0184
Gauteng
ZA
Telephone: 0128435196
email: m.kgatla@sanbi.org.za
homepage: http://fbip.co.za/contact/

Barbara van Asch
administrative point of contact
position: Senior Lecturer
Stellenbosch University
Stellenbosch University, Department of Genetics, JC Smuts Building Room 224
Stellenbosch University
7601
Western Cape
ZA
Telephone: 0218085888
email: bva@sun.ac.za
homepage: http://www.sun.ac.za/english/faculty/agri/genetics/staff-students/academic-staff