Chironomid Specimen records in the Chironomid DNA Barcode Database.

Study Extent

Chironomid species were captured in Japan and Tuvalu from 1989 to 2015.

Sampling

Specimens were collected in three major ways. (1) Larvae and pupae in bottom sediments were captured with a Ekman bottom grab sampler or a dip net in lentic waters and with a Surber sampler or a D-frame net in lotic waters. Aquatic weeds were also hand-collected for periphytic species. (2) A part of chironomids captured underwater was reared to adults in the laboratory. They were kept in water aerated with a pump in tanks. (3) Midges were collected with an emergence trap or a sweeping net on and around waters where they emerged.

Quality Control

Scientific names were validated against the Catalogue of the Insects of Japan Vol.8 (Yamamoto and Yamamoto 2014) and the GBIF Backbone Taxonomy.

Method steps

1. Morphological identification was used to determine the species names of specimens, along with DNA barcoding and ecological observations for a limited number of species. Geographic coordinates and uncertainties were measured using gazetteers such as Google Maps following procedures described in Chapman and Wieczorek (2006). Chironomid specimen records were extracted from the Chironomid DNA Barcode Database and organized into species occurrence data using a MySQL database.

1. Ablabesmyia
   rank: genus
2. Ainuyusurika
   rank: genus
3. Benthalia
   rank: genus
4. Biwatendipes
   rank: genus
5. Brillia
   rank: genus
6. Bryophaenocladius
   rank: genus
7. Cardiocladius
   rank: genus
8. Chironomus
   rank: genus
9. Cladopelma
   rank: genus
10. Cladotanytarsus
    rank: genus
11. Compterosmittia
    rank: genus
12. Conchapelopia
    rank: genus
13. Corynoneura
    rank: genus
14. Cricotopus
    rank: genus
15. Cryptochironomus
    rank: genus
16. Demicryptochironomus
    rank: genus
17. Diamesa
    rank: genus
18. Dicrotendipes
    rank: genus
19. Endochironomus
    rank: genus
20. Eukiefferiella
    rank: genus
21. Euryhapsis
    rank: genus
22. Glyptotendipes
    rank: genus
23. Harnischia
    rank: genus
24. Heleniella
    rank: genus
25. Heterotrissocladius
    rank: genus
26. Kiefferulus
    rank: genus
27. Limnophyes
    rank: genus
28. Lipiniella
    rank: genus
29. Metriocnemus
    rank: genus
30. Microchironomus
    rank: genus
31. Micropsectra
    rank: genus
32. Microtendipes
    rank: genus
33. Nanocladius
    rank: genus
34. Neozavrelia
    rank: genus
35. Nilodorum
    rank: genus
36. Nilotanypus
    rank: genus
37. Nilothauma
    rank: genus
38. Orthocladius
    rank: genus
39. Parachironomus
    rank: genus
40. Paracladopelma
    rank: genus
41. Parakiefferiella
    rank: genus
42. Paramerina
    rank: genus
43. Parametriocnemus
    rank: genus
44. Paraphaenocladius
    rank: genus
45. Paratanytarsus
    rank: genus
46. Paratendipes
    rank: genus
47. Polypedilum
    rank: genus
48. Potthastia
    rank: genus
49. Procladius
    rank: genus
50. Prodiamesa
    rank: genus
51. Propsilocerus
    rank: genus
52. Psectrocladius
    rank: genus
53. Psectrotanypus
    rank: genus
54. Pseudorthocladius
    rank: genus
55. Rheocricotopus
    rank: genus
56. Rheopelopia
    rank: genus
57. Rheotanytarsus
    rank: genus
58. Smittia
    rank: genus
59. Stempellinella
    rank: genus
60. Stenochironomus
    rank: genus
61. Stictochironomus
    rank: genus
62. Synorthocladius
    rank: genus
63. Tanypus
    rank: genus
64. Tanytarsus
    rank: genus
65. Telmatogeton
    rank: genus
66. Thienemanniella
    rank: genus
67. Thienemannimyia
    rank: genus
68. Trissopelopia
    rank: genus
69. Tvetenia
    rank: genus
70. Virgatanytarsus
    rank: genus
71. Yaetanytarsus
    rank: genus

Japan