The InBIO Barcoding Initiative Database: Portuguese Stag beetles (Coleoptera, Lucanidae)

Study Extent

Specimens were collected in ten localities of continental Portugal. Sampling was conducted from 2015 to 2019, in several field work expeditions.

Sampling

Specimens were collected in ten localities of continental Portugal. Sampling was conducted between 2015 and 2019, by direct search. Collected specimens were identified using a binocular stereoscopic microscope and stored in 96% ethanol for downstream molecular analysis.

Quality Control

To each specimen the morphological identification was contrasted with the results of the Blast of the newly generated DNA barcodes in BOLD Identification Engine.

Method steps

1. Sampling was conducted in continental Portugal from 2015 and 2019, and consisted in direct search. Specimens collected were stored in 96% ethanol. A tissue sample (leg) was removed from which DNA was extracted and the COI DNA barcode fragment was sequenced. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/).

This dataset is composed of data relating to 11 Lucanidae (Coleoptera) specimens. All specimens were determined to species level. Three of the four Stag beetle species known to occur in continental Portugal are represented in the dataset, with Dorcus parallelipipedus as the most represented species in the dataset, accounting for seven of the eleven specimens.

1. Animalia
   rank: kingdom
2. Arthropoda
   rank: phylum
3. Insecta
   rank: class
4. Coleoptera
   rank: order
5. Lucanidae
   rank: family

Continental Portugal