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Specimens of Huffmanela lusitana of the Collection of Pathology of IPMA

Citation

Ramos P (2016). Specimens of Huffmanela lusitana of the Collection of Pathology of IPMA. Version 1.3. IPMA - Instituto Português do Mar e da Atmosfera. Occurrence dataset https://doi.org/10.15468/x0z0xw accessed via GBIF.org on 2020-09-16.

Description

Specimens of Huffmanela lusitana sp n. are preserved, as laid eggs in epaxial and hypaxial muscles of pouting, Trisopterus luscus (L.), of the collection of Pathology of IPMA, either as frozen tissue or in neutral buffered formalin. Pouting was captured using bottom trawl and gillnets, at the North East Atlantic Coast, between Figueira da Foz and Cabo da Roca. Pathological findings, microscopic and ultrastructural characteristics, egg features, host-parasite interaction at muscle level and a new host on a new geographic area, all suggest that a new species is involved.

Sampling Description

Study Extent

Northeast Atlantic coast of Portugal, between Figueira da Foz and Cabo da Roca (Portugal)

Sampling

Infected pouting were captured using bottom trawl and gillnets. Morphological Huffmanela lusitana eggs were performed on wet-mounted slides without coverslip pressure. For Scanning electronic microscopy, eggs were rinsed by pipetting and and routinely processed. SEM photographs were taken with JEOL5200-LV electronic microscope.

Method steps

  1. Morphological Huffmanela lusitana eggs were performed on wet-mounted slides without coverslip pressure. For Scanning electronic microscopy, eggs were rinsed by pipetting and and routinely processed. SEM photographs were taken with JEOL5200-LV electronic microscope. Muscle samples of all specimens were collected and dehydrated in a graded series of ethanol and embedded in paraffin for histological procedures.

Taxonomic Coverages

This dataset is associated with the description of a new species Huffmanela lusitana Ramos, Carvalho, Rosa, Alexandre-Pires, Seixas & Esteves, 2016
  1. Animalia
    rank: kingdom
  2. Nematoda
    rank: phylum
  3. Adenophorea
    rank: class
  4. Trichocephalida
    rank: order
  5. Trichosomoididae
    rank: family
  6. Huffmanela
    rank: genus
  7. Huffmanela lusitana
    rank: species

Geographic Coverages

Northeast Atlantic coast of Portugal, between Figueira da Foz and Cabo da Roca (Portugal)

Bibliographic Citations

Contacts

Paula Ramos
originator
position: Researcher
Instituto Português do Mar e da Atmosfera
Rua Alfredo Magalhães Ramalho
Lisboa
1449-006 Lisboa
PT
email: pramos@ipma.pt
Paula Ramos
metadata author
position: Researcher
Instituto Português do Mar e da Atmosfera
Rua Alfredo Magalhães Ramalho
Lisboa
1449-006 Lisboa
PT
email: pramos@ipma.pt
Raquel Carvalho
principal investigator
position: Researcher
University of Trás-os-Montes e Alto Douro (UTAD)
Apartado 1013
Vila Real
5001-801 Vila Real
PT
email: rakel.karvalho@hotmail.com
Fernanda Rosa
principal investigator
position: Researcher
Instituto Superior de Agronomia
Tapada da Ajuda
Lisboa
1349-017 Lisboa
PT
email: fhjrosa@isa.ulisboa.pt
userId: http://orcid.org/0000-0003-2777-0059
Graça Alexandre-Pires
principal investigator
position: Researcher
Faculty of Veterinary Medicine, University of Lisbon
Avenida da Universidade Técnica
Lisboa
1300-477 Lisboa
email: gpires@fmv.ulisboa.pt
Fernanda Seixas
principal investigator
position: Researcher
University of Trás-os-Montes e Alto Douro (UTAD)
Apartado 1013
Vila Real
5001-801 Vila Real
PT
email: fseixas@utad.pt
Alexandra Esteves
principal investigator
position: Researcher
University of Trás-os-Montes e Alto Douro (UTAD)
Apartado 1013
Vila Real
5001-801 Vila Real
PT
email: alexe@utad.pt
Paula Ramos
administrative point of contact
position: Researcher
Instituto Português do Mar e da Atmosfera
Rua Alfredo Magalhães Ramalho
Lisboa
1449-006 Lisboa
PT
email: pramos@ipma.pt
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