The InBIO Barcoding Initiative Database: Portuguese Earwigs (Dermaptera)

Sónia Ferreira; Daniel Oliveira; Pedro Brito Lopes; Pedro Sousa; Pedro Beja

doi:10.15468/jups46

The InBIO Barcoding Initiative Database: Portuguese Earwigs (Dermaptera)

Citation

Ferreira S, Oliveira D, Lopes P B, Sousa P, Beja P (2020). The InBIO Barcoding Initiative Database: Portuguese Earwigs (Dermaptera). Version 1.5. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Occurrence dataset https://doi.org/10.15468/jups46 accessed via GBIF.org on 2020-09-16.

Description

The dataset contains 56 records of Earwigs (Dermaptera) species collected from 2006 to 2019 in continental Portugal. All captured specimens were identified to species level, belonging to ten of the fourteen species of Dermaptera present in Portugal. Specimens were detected and captured by direct search of the environment. Captured specimens were preserved in 96% ethanol. From each specimen one leg (tissue sample) was removed to be used for DNA extraction. Preserved specimens and DNA extracts are deposited in the within the InBIO Barcoding Initiative (IBI) collection at the CIBIO (Research Center in Biodiversity and Genetic Resources). Samples of each species were selected for DNA sequencing based on geographic provenance of the corresponding specimen. Sequencing of the 658 bp DNA barcodes was conducted within the InBIO Barcoding Initiative (IBI) and all DNA sequences were submitted to BOLD (Barcode of Life Data System) and GenBank databases.

Purpose

The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.

Sampling Description

Study Extent

Specimens of Dermaptera were captured in thirty sites, distributed across eleven districts of the country. Sampling was conducted from 2006 to 2019, in several field work expeditions.

Sampling

Specimens were captured from 2006 to 2019 in continental Portugal by direct search of thirty sampling sites. Captured insects were preserved in 96% ethanol and stored at the InBIO Barcoding Initiative reference collection for downstream molecular analysis. Collected specimens were morphologically identified using a binocular stereoscopic microscope.

Quality Control

To each specimen the morphological identification was contrasted with the results of the Blast of the newly generated DNA barcodes in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/).

Method steps

Specimens of Dermaptera were captured in continental Portugal from 2006 and 2019 by direct search of the sampling site. Collected specimens were preserved in 96% ethanol prior to storage.
The specimens were morphologically identified resorting to available literature (Chopard, 1922, 1943; Lapeira and Pascual, 1980; Albouy and Caussanel, 1990; Bivar de Sousa, 1997).
Six specimens from each species (or all specimens, when less than six available) were selected for DNA extraction and sequencing, based on geographical location. For this, one leg was removed from each individual from which a 658 bp COI DNA barcode fragment was extracted, amplified and sequenced.
All obtained sequences were submitted to BOLD and GenBank databases and to each sequenced specimen the morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcodes in BOLD Identification Engine.
Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/).

Taxonomic Coverages

This dataset is composed of data relating to 56 Dermaptera specimens belonging to ten species present in Portugal. The four families of Dermaptera known to occur in Portugal are represented in the dataset, with all species of Spongiphoridae and Labiduridae known from the country fully represented. The most quantitatively represented species is Forficula auricularia with fifteen records.

Animalia
rank: kingdom
Arthropoda
rank: phylum
Insecta
rank: class
Dermaptera
rank: order
Anisolabididae
rank: family
Forficulidae
rank: family
Labiduridae
rank: family
Spongiphoridae
rank: family

Geographic Coverages

Continental Portugal.

Bibliographic Citations

Albouy, V. and Caussanel, C. (1990) Dermaptères ou Perc-Oreilles, Faune de France. Federation Francaise des Societes de Sciences Naturelles. -
Bivar de Sousa, A. (1997) ‘Dermápteros (Insecta: Dermaptera) novos ou pouco conhecidos para Portugal continental’, Boletim da Sociedade Portuguesa de Entomologia, 167(VI–17), pp. 229–244. -
Chopard, L. (1922) 3. Orthoptéres et Dermaptères, Faune de France. Paris: Office Central de Faunistique. -
Chopard, L. (1943) I. Orthoptèroïdes de l’Afreique du Nord, Faune de L’Empire Français. Paris. -
Lapeira, A. and Pascual, F. (1980) ‘Estudio preliminar de los Dermápteros de la Fauna Ibérica’, Trabajo Monografíco del Departamento de Zoología de la Universidad de Granada, 3(4), pp. 53–99. -

Contacts

Sónia Ferreira
originator
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: hiporame@gmail.com
userId: http://orcid.org/0000-0002-6884-3966

Daniel Oliveira
originator
position: Master’s Student
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
email: daniel.jet100@gmail.com
userId: http://orcid.org/0000-0002-8733-4104

Pedro Brito Lopes
originator
position: Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: pedro.lopes275@gmail.com
userId: http://orcid.org/0000-0002-5078-0602

Pedro Sousa
originator
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: psousa@cibio.up.pt
userId: http://orcid.org/0000-0002-5859-9656

Pedro Beja
originator
position: Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: pbeja@cibio.up.pt
userId: http://orcid.org/0000-0001-8164-0760

Sónia Ferreira
metadata author
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
PT
Telephone: +351 252660411
email: hiporame@gmail.com
userId: http://orcid.org/0000-0002-6884-3966

Daniel Oliveira
metadata author
position: Master’s Student
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: daniel.jet100@gmail.com
userId: http://orcid.org/0000-0002-8733-4104

Pedro Brito Lopes
metadata author
position: Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: pedro.lopes275@gmail.com
userId: http://orcid.org/0000-0002-5078-0602

Pedro Sousa
metadata author
position: Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: psousa@cibio.up.pt
userId: http://orcid.org/0000-0002-5859-9656

Pedro Beja
metadata author
position: Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: pbeja@cibio.up.pt
userId: http://orcid.org/0000-0001-8164-0760

Pedro Beja
administrative point of contact
position: Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: pbeja@cibio.up.pt
userId: http://orcid.org/0000-0001-8164-0760

Sónia Ferreira
administrative point of contact
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: hiporame@gmail.com
userId: http://orcid.org/0000-0002-6884-3966