We’re sorry, but GBIF doesn’t work properly without JavaScript enabled.
Our website has detected that you are using an outdated insecure browser that will prevent you from using the site. We suggest you upgrade to a modern browser.
{{nav.loginGreeting}}
  • Get data

      • Occurrences
      • Species
      • Datasets
      • Trends
  • How-to

    • Share data

      • Quick-start guide
      • Dataset classes
      • Data hosting
      • Standards
      • Become a publisher
      • Data quality
      • Data papers

    • Use data

      • Featured data use
      • Citation guidelines
      • GBIF citations
      • Citation widget
  • Tools

    • Publishers

      • IPT
      • Data validator
      • Suggest a dataset
      • Scientific Collections

    • Users

      • GBIF API
      • Data processing
      • rgbif
      • MAXENT
      • Tools catalogue

    • GBIF labs

      • Species matching
      • Name parser
      • Sequence ID
      • Relative observation trends
      • GBIF data blog
  • Community

    • Network

      • Participants
      • Nodes
      • Publishers
      • Network contacts
      • Community forum
      • alliance for biodiversity knowledge

    • Volunteers

      • Mentors
      • Ambassadors
      • Translators
      • Citizen scientists

    • Activities

      • Capacity enhancement
      • Training and e-Learning
      • Programmes & projects
      • Living Atlases
  • About

    • Inside GBIF

      • What is GBIF?
      • Become a member
      • Governance
      • Funders
      • Partnerships
      • Implementation plan
      • Contacts

    • News & outreach

      • News
      • Newsletters and lists
      • Events
      • Ebbe Nielsen Challenge
      • Young Researchers Award
      • Science Review
  • User profile

The InBIO Barcoding Initiative Database: contribution to the knowledge on DNA barcodes of Iberian Plecoptera

Dataset homepage

Citation

Ferreira S, Tierno de Figueroa J M, Martins F M S, Veríssimo J, Quaglietta L, Grosso-Silva J M, Lopes P B, Sousa P, Beja P (2020). The InBIO Barcoding Initiative Database: contribution to the knowledge on DNA barcodes of Iberian Plecoptera. Version 1.4. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Occurrence dataset https://doi.org/10.15468/3vkdr2 accessed via GBIF.org on 2020-09-16.

Description

The dataset contains 71 records of Plecoptera species collected from 2004 to 2018 in the Iberian Peninsula. All specimens have been morphologically identified to species level, and belong to 29 species in total. Specimens were captured during fieldwork directed specifically for the sampling of Plecoptera using different methodologies and stored in 96% ethanol. A tissue sample, usually a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) online database. Preserved specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).

Purpose

The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.

Sampling Description

Study Extent

Iberian Peninsula.

Sampling

The studied material was collected in 40 different localities from Iberian Peninsula. Sampling was conducted between 2004 and 2018 on a wide range of habitats, using mainly hand-held sweep-nets or direct search for specimens. Collected specimens were examined in ethanol using a binocular stereoscopic microscope, and they were stored in 96% ethanol for downstream molecular analysis. Morphological identification was performed using keys and descriptions from literature (mainly Tierno de Figueroa et al. 2003 , Tierno de Figueroa et al. 2015).

Quality Control

All DNA barcodes sequences were compared against the BOLD database and the 99 top hits were inspected in order to detect possible issues due to contaminations or misidentifications. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org).

Method steps

  1. Specimens were collected in 40 different localities of Iberian Peninsula. Sampling was conducted from 2004 to 2018, and consisted of direct search of specimens on rocks and vegetation of streams and river margins, and in the use of entomological nets to intercept specimens flight. Specimens collected were stored in 96% ethanol. A tissue sample (leg) was removed, from which DNA was extracted and the COI DNA barcode fragment was sequenced. Data generated were submitted to BOLD, GenBank and GBIF.

Taxonomic Coverages

This dataset is composed of data relating to 71 Plecoptera specimens. All specimens were determined to species level. Overall, 29 species are represented in the dataset. These species belong to 16 genera and 7 families.
  1. Animalia
    rank: kingdom
  2. Arthropoda
    rank: phylum
  3. Insecta
    rank: class
  4. Plecoptera
    rank: order
  5. Capniidae
    rank: family
  6. Chloroperlidae
    rank: family
  7. Leuctridae
    rank: family
  8. Nemouridae
    rank: family
  9. Perlidae
    rank: family
  10. Perlodidae
    rank: family
  11. Taeniopterygidae
    rank: family

Geographic Coverages

Iberian Peninsula

Bibliographic Citations

  1. Tierno de Figueroa J, Sánchez-Ortega A, Membiela-Iglesia P, Luzón-Ortega J (2003). Plecoptera. Fauna Ibérica. CSIC, Madrid, 404 pp. -
  2. Tierno de Figueroa J, Luzón-Ortega J, López-Rodríguez M (2015) Orden Plecoptera. In: IBERFAUNA. El Banco de Datos de la Fauna Ibérica. Museo Nacional de Ciencias Naturales (CSIC). http://iberfauna.mncn.csic.es/showficha.aspx?rank=J&idtax=571. Accessed on: 2020-4-09. - http://iberfauna.mncn.csic.es/showficha.aspx?rank=J&idtax=571

Contacts

Sónia Ferreira
originator
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
PT
Telephone: +351 252660411
email: hiporame@gmail.com
userId: http://orcid.org/0000-0002-6884-3966
J. Manuel Tierno de Figueroa
originator
position: Full Professor
Universidad de Granada
ES
userId: http://orcid.org/0000-0003-1616-9815
Filipa M S Martins
originator
position: PhD student
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
PT
email: filipamsmartins@gmail.com
userId: http://orcid.org/0000-0003-4191-5031
Joana Veríssimo
originator
position: PhD student
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
PT
email: joana.filipa.verissimo@gmail.com
userId: http://orcid.org/0000-0002-8721-9376
Lorenzo Quaglietta
originator
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
PT
email: lorenzo.quaglietta@cibio.up.pt
userId: http://orcid.org/0000-0002-9703-0171
José M. Grosso-Silva
originator
position: Entomology Curator
Museu de História Natural e da Ciência da Universidade do Porto
PT
email: jmgrossosilva@gmail.com
userId: http://orcid.org/0000-0002-1094-0771
Pedro Brito Lopes
originator
position: Researcher
Rua do Torgal, nº 16 (Trigais)
Covilhã
PT
userId: http://orcid.org/0000-0002-5078-0602
Pedro Sousa
originator
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
PT
Telephone: +351 252660411
email: psousa@cibio.up.pt
userId: http://orcid.org/0000-0002-5859-9656
Pedro Beja
originator
position: Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
PT
Telephone: +351 252660411
email: pbeja@cibio.up.pt
userId: http://orcid.org/0000-0001-8164-0760
Sónia Ferreira
metadata author
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
PT
Telephone: +351 252660411
email: hiporame@gmail.com
userId: http://orcid.org/0000-0002-6884-3966
J. Manuel Tierno de Figueroa
metadata author
position: Full Professor
Universidad de Granada
ES
email: jmtdef@ugr.es
userId: http://orcid.org/0000-0003-1616-9815
Filipa M S Martins
metadata author
position: PhD student
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
PT
email: filipamsmartins@gmail.com
userId: http://orcid.org/0000-0003-4191-5031
Joana Veríssimo
metadata author
position: PhD student
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
PT
email: joana.filipa.verissimo@gmail.com
userId: http://orcid.org/0000-0002-8721-9376
Lorenzo Quaglietta
metadata author
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
PT
Telephone: +351 252660411
email: lorenzo.quaglietta@cibio.up.pt
userId: http://orcid.org/0000-0002-9703-0171
José M. Grosso-Silva
metadata author
position: Entomology Curator
Museu de História Natural e da Ciência da Universidade do Porto
PT
email: jmgrossosilva@gmail.com
userId: http://orcid.org/0000-0002-1094-0771
Pedro Brito Lopes
metadata author
position: Researcher
Rua do Torgal, nº 16 (Trigais)
Covilhã
PT
email: pedro.lopes275@gmail.com
userId: http://orcid.org/0000-0002-5078-0602
Pedro Sousa
metadata author
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
PT
Telephone: +351 252660411
email: psousa@cibio.up.pt
userId: http://orcid.org/0000-0002-5859-9656
Pedro Beja
metadata author
position: Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
PT
Telephone: +351 252660411
email: pbeja@cibio.up.pt
userId: http://orcid.org/0000-0001-8164-0760
Pedro Beja
administrative point of contact
position: Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
PT
Telephone: +351 252660411
email: pbeja@cibio.up.pt
userId: http://orcid.org/0000-0001-8164-0760
Sónia Ferreira
administrative point of contact
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
PT
Telephone: +351 252660411
email: hiporame@gmail.com
userId: http://orcid.org/0000-0002-6884-3966
What is GBIF? API FAQ Newsletter Privacy Terms and agreements Citation Code of Conduct Acknowledgements
Contact GBIF Secretariat Universitetsparken 15 DK-2100 Copenhagen Ø Denmark