EDP Baixo Sabor: Phytoplankton - Ecological Monitoring Program [2016]

Study Extent

The study was carried out in one sampling station, 1300 m upstream of the dam, in the deepest zone of the Baixo Sabor reservoir. Six sampling campaigns took place between 3 March and 22 November 2016, covering winter, spring, summer (3 samples) and autumn.

Sampling

Sampling followed the procedures defined by the Portuguese National Water Authority in the protocol for sampling and analysis of phytoplankton quality element under the Water Framework Directive. In each sampling campaign, a two-step procedure was adopted. First (a) a water column profile for temperature and dissolved oxygen was perform using a multiparametric probe (b) the water transparency was measure using a Secchi disk (Secchi depth), and (c) the depth of the euphotic zone was estimated (2.5 x the Secchi Depth). Second, (a) water samples were collected, using an integrated water sampling device (hydrobios), from the surface to euphotic zone maximum depth; (b) samples were preserved in loco with Alkaline Lugol’s solution and transported in 48 hours to the laboratory. The samples were stored in a dark and cold place (1º to 4ºC) for a maximum period of 6 months. They were periodically examined and recompleted with Alkaline Lugol’s solution, whenever needed. In the lab, the analysis for identification (to the lower possible taxonomic level), quantification (cells/mL) and biovolume (mm3/m3) were carried out using sedimentation chambers and an inverted microscope (Utermöhl technique), as described in EN 15204:2006 (Water quality - Guidance standard on the enumeration of phytoplankton using inverted microscopy (Utermöhl technique)) and EN 16695:2015 (Water quality - Guidance on the estimation of phytoplankton biovolume).

Quality Control

Sampling method and laboratory identification, quantification and biovolume estimation are accredited (accreditation certificate and technical annex L0216) by Instituto Português de Acreditação (IPAC), as demanded by ISO/IEC 17025:2017 (General requirements for the competence of testing and calibration laboratories). All individuals were identified by experts with a deep knowledge of Phytoplankton taxonomy.

Method steps

1. To be completed

The taxonomic coverage of this dataset includes 6 kingdoms, 15 phylum, 17 classes, 48 orders, 77 families, 144 genus and 305 species of the list of the EU’s Water Directive framework. The actual observations encompass 5 kingdoms, 9 phylum, 10 classes, 18 orders, 23 families, 30 genus and 31 species. The identification was made, whenever possible, to species level; otherwise, the phylum, class, order, family or genus were specified.

1. Animalia
   rank: kingdom
2. Bacteria
   rank: kingdom
3. Chromista
   rank: kingdom
4. Eubacteria
   rank: kingdom
5. Plantae
   rank: kingdom
6. Protozoa
   rank: kingdom
7. Cnidaria
   rank: phylum
8. Cyanobacteria
   rank: phylum
9. Eubacteria
   rank: phylum
10. Bacillariophyta
    rank: phylum
11. Chrysophyta
    rank: phylum
12. Cryptophyta
    rank: phylum
13. Haptophyta
    rank: phylum
14. Myzozoa
    rank: phylum
15. Ochrophyta
    rank: phylum
16. Charophyta
    rank: phylum
17. Chlorophyta
    rank: phylum
18. Chromophyta
    rank: phylum
19. Choanozoa
    rank: phylum
20. Euglenophyta
    rank: phylum
21. Euglenozoa
    rank: phylum
22. Hydrozoa
    rank: class
23. Cyanophyceae
    rank: class
24. Bacillariophyceae
    rank: class
25. Cryptophyceae
    rank: class
26. Prymnesiophyceae
    rank: class
27. Dinophyceae
    rank: class
28. Bacillariophyceae
    rank: class
29. Chrysophyceae
    rank: class
30. Xanthophyceae
    rank: class
31. Klebsormidiophyceae
    rank: class
32. Zygnematophyceae
    rank: class
33. Chlorophyceae
    rank: class
34. Pedinophyceae
    rank: class
35. Prasinophyceae
    rank: class
36. Trebouxiophyceae
    rank: class
37. Bacillariophyceae
    rank: class
38. Choanoflagellatea
    rank: class
39. Euglenoidea
    rank: class
40. Anthoathecata
    rank: order
41. Chroococcales
    rank: order
42. Nostocales
    rank: order
43. Chroococcales
    rank: order
44. Bacillariales
    rank: order
45. Cryptomonadales
    rank: order
46. Pyrenomonadales
    rank: order
47. Prymnesiales
    rank: order
48. Gonyaulacales
    rank: order
49. Gymnodiniales
    rank: order
50. Peridiniales
    rank: order
51. Suessiales
    rank: order
52. Achnanthales
    rank: order
53. Aulacoseirales
    rank: order
54. Bacillariales
    rank: order
55. Coscinodiscales
    rank: order
56. Cymbellales
    rank: order
57. Eunotiales
    rank: order
58. Fragilariales
    rank: order
59. Mastogloiales
    rank: order
60. Melosirales
    rank: order
61. Naviculales
    rank: order
62. Rhizosoleniales
    rank: order
63. Rhopalodiales
    rank: order
64. Surirellales
    rank: order
65. Tabellariales
    rank: order
66. Thalassiophysales
    rank: order
67. Thalassiosirales
    rank: order
68. Chromulinales
    rank: order
69. Ochromonadales
    rank: order
70. Synurales
    rank: order
71. Mischococcales
    rank: order
72. Oscillatoriales
    rank: order
73. Synechococcales
    rank: order
74. Klebsormidiales
    rank: order
75. Zygnematales
    rank: order
76. Chlamydomonadales
    rank: order
77. Sphaeropleales
    rank: order
78. Tetrasporales
    rank: order
79. Volvocales
    rank: order
80. Pedinomonadales
    rank: order
81. Chlorodendrales
    rank: order
82. Pyramimonadales
    rank: order
83. Chlorellales
    rank: order
84. Oocystales
    rank: order
85. Trebouxiophyceae ordo incertae sedis
    rank: order
86. Pennales
    rank: order
87. Craspedida
    rank: order
88. Euglenida
    rank: order
89. Cladonematidae
    rank: order
90. Chroococcaceae
    rank: family
91. Synechococcaceae
    rank: family
92. Nostocaceae
    rank: family
93. Oscillatoriaceae
    rank: family
94. Rivulariaceae
    rank: family
95. Chroococcales
    rank: family
96. Bacillariaceae
    rank: family
97. Cryptomonadaceae
    rank: family
98. Chroomonadaceae
    rank: family
99. Pyrenomonadaceae
    rank: family
100. Chrysochromulinaceae
     rank: family
101. Ceratiaceae
     rank: family
102. Kareniaceae
     rank: family
103. Phytodiniaceae
     rank: family
104. Glenodiniaceae
     rank: family
105. Peridiniaceae
     rank: family
106. Borghiellaceae
     rank: family
107. Achnanthaceae
     rank: family
108. Achnanthidiaceae
     rank: family
109. Cocconeidaceae
     rank: family
110. Aulacoseiraceae
     rank: family
111. Bacillariaceae
     rank: family
112. Hemidiscaceae
     rank: family
113. Cymbellaceae
     rank: family
114. Gomphonemataceae
     rank: family
115. Eunotiaceae
     rank: family
116. Peroniaceae
     rank: family
117. Fragilariaceae
     rank: family
118. Mastogloiaceae
     rank: family
119. Melosiraceae
     rank: family
120. Diploneidaceae
     rank: family
121. Naviculaceae
     rank: family
122. Neidiaceae
     rank: family
123. Pinnulariaceae
     rank: family
124. Pleurosigmataceae
     rank: family
125. Stauroneidaceae
     rank: family
126. Rhizosoleniaceae
     rank: family
127. Rhopalodiaceae
     rank: family
128. Surirellaceae
     rank: family
129. Tabellariaceae
     rank: family
130. Catenulaceae
     rank: family
131. Skeletonemataceae
     rank: family
132. Stephanodiscaceae
     rank: family
133. Thalassiosiraceae
     rank: family
134. Dinobryaceae
     rank: family
135. Chromulinaceae
     rank: family
136. Mallomonadaceae
     rank: family
137. Pleurochloridaceae
     rank: family
138. Ammatoideaceae
     rank: family
139. Oscillatoriaceae
     rank: family
140. Synechococcaceae
     rank: family
141. Elakatotrichaceae
     rank: family
142. Closteriaceae
     rank: family
143. Desmidiaceae
     rank: family
144. Zygnemataceae
     rank: family
145. Golenkiniaceae
     rank: family
146. Micractiniaceae
     rank: family
147. Sphaeropleaceae
     rank: family
148. Treubariaceae
     rank: family
149. Volvocaceae
     rank: family
150. Characiaceae
     rank: family
151. Hydrodictyaceae
     rank: family
152. Radiococcaceae
     rank: family
153. Scenedesmaceae
     rank: family
154. Schroederiaceae
     rank: family
155. Selenastraceae
     rank: family
156. Sphaeropleales incertae sedis
     rank: family
157. Sphaerocystidaceae
     rank: family
158. Chlamydomonadaceae
     rank: family
159. Goniaceae
     rank: family
160. Pedinomonadaceae
     rank: family
161. Chlorodendraceae
     rank: family
162. Pyramimonadaceae
     rank: family
163. Chlorellaceae
     rank: family
164. Oocystaceae
     rank: family
165. Trebouxiophyceae incertae sedis
     rank: family
166. Salpingoecaceae
     rank: family
167. Euglenaceae
     rank: family

The data were collected in a single point in the Baixo Sabor reservoir, at a fixed station 1300 m upstream of the dam in the deeper zone of the reservoir.