Nocardioides silvaticus Li, Shi, Zhang, and Wang 2019
- Dataset
- Nocardioides silvaticus sp. nov., isolated from forest soil
- Rank
- SPECIES
- Published in
- Li, Chan, Shi, Kaixiang, Zhang, Yuxiao, Wang, Gejiao (2019): Nocardioides silvaticus sp. nov., isolated from forest soil. International Journal of Systematic and Evolutionary Microbiology 69 (1): 68-73, DOI: 10.1099/ijsem.0.003079, URL: http://dx.doi.org/10.1099/ijsem.0.003079
Classification
- kingdom
- Bacteria
- phylum
- Actinobacteria
- class
- Actinobacteridae
- order
- Actinomycetales
- family
- Nocardioidaceae
- genus
- Nocardioides
- species
- Nocardioides silvaticus
description
Cells are Gram-stain-positive, strictly aerobic, rod-shaped, approximately 0.8 – 1.3 µm long and 0.3 – 0.4 µm wide, and non-motile. Colonies grown on R 2 A agar are creamcoloured, circular, smooth, raised and 1 – 2 mm in diameter after incubation for 3 days. The growth temperature and pH range on R 2 A agar are 20 – 42 Ǫ C (optimum, 37 Ǫ C) and pH 7 – 9 (optimum, pH 7). It can tolerate salt up to 5 % NaCl (optimum without addition of NaCl). Catalase is positive but oxidase is negative. Negative for H 2 S production, indole production, methyl red production and Voges – Proskauer test. Hydrolyses casein and DNA, but not gelatin, starch, cellulose, Tween 20, Tween 40, Tween 60 and Tween 80. As sole carbon sources, sucrose, fructose, cysteine, propionate, valerate, 3 - hydroxybutyric acid, acetate, 3 - hydroxy benzoate, L- serine, potassium gluconate and aesculin ferric citrate are utilized, but maltose, arabinose, glucose, celllobiose, rhamnose, sorbose, D- galactose, mannitol, sorbitol, D- ribose, seignette salt, L- proline, L- arginine, sarcosine, malic acid, sodium citrate and phenylalaline are not. For acid production, D- ribose, arabinose and xylose are positive, but glucose, cellobiose, rhamnose, sucrose, ribose, fructose, lactose, D-galactose, maltose, mannitol, sorbitol, sorbose and inositol are negative. Positive for alkaline phosphatase, esterase C 4, esterase lipaes C 8, valine arylamidase, leucine arylamidase, cysteine, trypsin, acid phosphatase, naphthol-AS-BI-phosphohydrolase, b- glucosidase and a- glucosidase, but negative for lipase C 14, a - chymotrypsin, a- galactosidase, b-galactosidase, b- glucuronidase, N - acetyl-b- glucosaminidase, a- mannosidase and a- fucosidase activities. The main respiratory quinone is meanquinone- 8 (H 4). The major components of cellular fatty acids are iso-C, C 17: 1! 8 c, C, C 17: 1! 6 c and C 17: 0 10 - methyl (TBSA). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid. The cellwall peptidoglycan contains LL- diaminopimelic acid. 16: 0 17: 0 The type strain is S- 34 T (= KCTC 49137 T = CCTCC AB 2018079 T), isolated from soil at Xinxiang Forest Ecology Park in Henan Province, PR China. The DNA G + C content of strain S- 34 T was 71.2 %. The accession numbers of the 16 S rRNA gene sequence and genome sequence of strain S- 34 T are MH 341589 and QGDD 00000000, respectively.
etymology
Nocardioides silvaticus sp. nov. (sil. va′ ti. cus. L. masc. adj. silvaticus belonging to a forest).