Limnichthys koreanus, a new species of creediid fish from South Korea (Teleostei: Acropomatiformes: Creediidae)

Study Extent

Twelve specimens of the new species (35.8–45.3 mm TL, 33.4–40.0 mm SL) were collected from subtidal zones at depths between 1–5m in Seogwipo, Jeju Island, Korea between July 2022 and December 2023.Tissue samples were taken from the right side of the body, preserved in 99% ethanol, and deposited in the in the Marine Fish Resource Bank of Korea (MFRBK). We followed the immersion specimen production manual of the Marine Fish Resource Bank of Korea (MFRBK), under the National Institute of Marine Biological Resources (MABIK) of the Ministry of Oceans and Fisheries (MOF).

Sampling

using scoop net

Method steps

1. Methods for counting measuring follow Waite (1904), Nelson (1978), and Yoshino et al. (1999). Images of specimens were taken using a digital camera (D750; Nikon, Japan). Detailed morphological characteristics were examined with a stereomicroscope (SZX-16; Olympus, Tokyo, Japan). Osteological details were determined from X-ray images, cleared and stained specimens, and micro-CT data. Micro-CT scans were taken using Phoenix V-Tome-X C450 Volume Graphics® VGSTUDIO Max software and handled using Volume Graphics® myVGL viewer. Mitochondrial cytochrome c oxidase subunit I (COI) and 16S ribosomal RNA (16S) were amplified from extracted gDNA. Sequences were read by BigDye Terminator v3.1 cycle sequencing kits (Applied Biosystems, Foster City, CA, USA) with an ABI PRISM 3730XL analyzer (96 capillary type; Applied Biosystems).

32.2225/126.24191 to 33.4602/126.93391