NDFF Environmental DNA

Ronald Laan

doi:10.15468/raajkb

NDFF Environmental DNA

Citation

Laan R, Oomen D (2018). NDFF Environmental DNA. Dutch National Database of Flora and Fauna (NDFF). Metadata dataset https://doi.org/10.15468/raajkb accessed via GBIF.org on 2020-07-12.

Description

Environmental DNA (eDNA) is a DNA-based research method used to establish the presence of a species in samples. The underlying principle is that all organisms living in water emit DNA via skin cells, faeces and urine. The presence of a target species can be established by collecting water samples and analysing them for DNA in a laboratory. Research has shown that DNA dissolves in water within 2 to 3 weeks. When a water sample indicates the presence of DNA of a species, this means habitation must have been recent. Detection is carried out via a Polymerase Chain Reaction (PCR) in a laboratory , using species-specific primers. In all cases, a large number (8 to 12) of PCR reactions are carried out on each sample (replica samples). The number of reactions that indicate a positive result for the DNA of the target species indicates how much DNA the sample contains. The amount of DNA is causally related to the occurrence of the target species, but there are many factors that affect the disintegration and production of eDNA. Dataset available via https://www.ndff.nl/english / serviceteamndff@natuurloket.nl Positive observations without zeros. 1,340 records (March 2018) NDFF protocol https://www.ndff.nl/overdendff/validatie/protocollen/10-002-environmental-dna-onderzoek/

Taxonomic Coverages

all organisms living in water detectable by DNA via skin cells, faeces and urine.

Amphibia
common name: Amphibian rank: class
Misgurnus fossilis
common name: European weatherfish rank: species

Geographic Coverages

Netherlands

Bibliographic Citations

Contacts

Ronald Laan
originator
position: datamanager
RAVON
NL
homepage: http://ravon.nl/English/tabid/1599/Default.aspx

Dries Oomen
metadata author
position: metadata publisher
NDFF
NL
email: serviceteamNDFF@natuurloket.nl
homepage: https://www.ndff.nl/english/

Henk de Vries
distributor
position: Node manager NLBIF
NLBIF Netherlands Biodiversity Information Facility
P.O.Box 9517
Leiden
2300 RA
NL
email: henk.devries@naturalis.nl

Dries Oomen
administrative point of contact
position: metadata publisher
NDFF
NL
email: serviceteamNDFF@natuurloket.nl
homepage: https://www.ndff.nl/english