The InBIO Barcoding Initiative Database: DNA Barcoding reveals sexual dimorphism in Isotrias penedana Trematerra, 2013 (Lepidoptera: Tortricidae, Chlidanotinae)

Study Extent

Specimens were collected in two different localities of northerner continental Portugal. Sampling was conducted from 2012 to 2016.

Sampling

Sampling of specimens was done at two different localities of continental Portugal. Sampling was conducted between 2012 to 2016. Captured specimens were kept in dry storage in the Research Collection of Martin Corley, while DNA extracts were stored in 96% ethanol at the InBIO Barcoding Initiative reference collection for downstream molecular analysis. The collected specimens were morphologically identified.

Quality Control

The DNA barcoded specimen morphological identification was contrasted with the results of the Blast of the newly generated DNA barcode in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).

Method steps

1. Sampling of specimens was done at two different localities of northern continental Portugal, between 2012 and 2016. Specimens were detected and captured by direct search with an hand-netted during the afternoon, and by night using mercury-vapor lamps as lures.
2. The captured specimens were preserved in dry storage.
3. The specimen was morphologically identified using a binocular stereoscopic microscope and resorting to available literature (Corley & Ferreira, 2017, Razowski, 2002; Trematerra, 1991, 2013).
4. DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual from which a 658 bp COI DNA barcode fragment was extracted, amplified and sequenced.
5. The obtained sequences were submitted to BOLD and GenBank databases and the specimens’ morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcode in BOLD Identification Engine.
6. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).

This dataset is composed of data relating to 5 Lepidoptera specimens. All specimens were determined to species level. All specimens belong to two species of the genus Isotrias Meyrick, 1895 and the family Tortricidae.

1. Animalia
   rank: kingdom
2. Arthropoda
   rank: phylum
3. Insecta
   rank: class
4. Lepidoptera
   rank: order
5. Tortricidae
   rank: family

Continental Portugal.