The InBIO Barcoding Initiative Database: Ypsolopha rhinolophi sp. nov. (Lepidoptera: Ypsolophidae), a new species from Portugal and France unveiled by bats

Martin Corley; Sónia Ferreira; Vanessa Alves Mata; Pedro Sousa; Pedro Beja

doi:10.15468/zbu2w8

The InBIO Barcoding Initiative Database: Ypsolopha rhinolophi sp. nov. (Lepidoptera: Ypsolophidae), a new species from Portugal and France unveiled by bats

Citation

Corley M, Ferreira S, Mata V A, Sousa P, Beja P (2021). The InBIO Barcoding Initiative Database: Ypsolopha rhinolophi sp. nov. (Lepidoptera: Ypsolophidae), a new species from Portugal and France unveiled by bats. Version 1.2. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Occurrence dataset https://doi.org/10.15468/zbu2w8 accessed via GBIF.org on 2021-09-21.

Description

The dataset contains eight records of moth specimens of the genus Ypsolopha collected from 2015 to 2017 in continental Portugal. The study of these specimens resulted in the description of a new species, Y. rhinolophi (Corley, 2019), present in northern Portugal and south-east France, similar to Y. alpella (Denis & Schiffermüller, 1775) and Y. lucella (Fabricius, 1775), based on clear differences in male and female genitalia and on DNA barcodes in Corley et al. (2019). Specimens were detected and captured by direct search of the environment during the day, with adults hand-netted and larvae reared to adulthood, and by night using mercury-vapor lamps to attract the insects. Captured specimens were identified to species level and preserved in 96% ethanol. A tissue sample, usually a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) and GenBank online databases. Preserved specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).

Purpose

The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.

Sampling Description

Study Extent

Specimens were collected in seven different localities of continental Portugal. Sampling was conducted from 2015 to 2017.

Sampling

Sampling of specimens was done at seven different localities of continental Portugal. Sampling was conducted between 2015 to 2017. Captured specimens were preserved in 96% ethanol and stored at the InBIO Barcoding Initiative reference collection for downstream molecular analysis. The collected specimens were morphologically identified.

Quality Control

The DNA barcoded specimen morphological identification was contrasted with the results of the Blast of the newly generated DNA barcode in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).

Method steps

Sampling of specimens was done at seven different localities of continental Portugal, between 2015 and 2017. Specimens were detected and captured by direct search during the day, with adults hand-netted and larvae reared to adulthood, and by night using mercury-vapor lamps as lures.
The captured specimen was preserved in 96% ethanol.
The specimen was morphologically identified using a binocular stereoscopic microscope and resorting to available literature (Clerck, 1759; Corley, Ferreira & Mata, 2019; Denis & Schiffermüller, 1775; Fabricius, 1775, 1787; Jin, Wang & Li, 2013; Linnaeus, 1761).
DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual from which a 658 bp COI DNA barcode fragment was extracted, amplified and sequenced.
The obtained sequences were submitted to BOLD and GenBank databases and the specimens’ morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcode in BOLD Identification Engine.
Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).

Taxonomic Coverages

This dataset is composed of data relating to 8 Lepidoptera specimens. All specimens were determined to species level. All specimens belong to the genus Ypsolopha Latreille, 1796 and the family Ypsolophidae.

Animalia
rank: kingdom
Arthropoda
rank: phylum
Insecta
rank: class
Lepidoptera
rank: order
Ypsolophidae
rank: family

Geographic Coverages

Continental Portugal.

Bibliographic Citations

Clerck, C.A. (1759) Icones insectorum rariorum cum nominibus eorum trivialibus locisque e C. Linnaei Systemae Naturae allegatis, Stockholm, 21 pp., 55 pls. -
Corley, M.F.V., Ferreira, S. & Mata, V.A. (2019). Ypsolopha rhinolophi sp. nov. (Lepidoptera: Ypsolophidae), a new species from Portugal and France unveiled by bats. Zootaxa, 4609(3): 565–573. - https://doi.org/10.11646/zootaxa.4609.3.10
Denis, M. & Schiffermüller, I. (1775) Ankündung eines systematischen Werkes von den Schmetterlingen der Wienergegend. Vienna, 324 pp. -
Fabricius, J.C. (1775) Systema Entomologicae, sistens Insectorum classes, ordines, genera, species, adjectis, synonymis, locis, descriptionibus, observationibus. Flensburg, Lipsia, 832 pp. - https://doi.org/10.5962/bhl.title.36510
Fabricius, J.C. (1787) Mantissa Insectorum sistens eorum species, nuper detectas adjectis characteribus generis, differentiis specificis emendationibus, observationibus. Vol. 2. Impensis Christ. Gottl. Proft, Hafniae, 382 pp. - https://doi.org/10.5962/bhl.title.11657
Jin, Q., Wang, S.X. & Li, H.H. (2013) Review of the genus Ypsolopha Latreille, 1796 from China. Zootaxa, 3705 (1), 1–91. - https://doi.org/10.11646/zootaxa.3705.1.1
Linnaeus, C. (1761) Fauna Svecica. 2nd Edition. Laurentii Salvii, Stockholm, 578 pp. -

Contacts

Martin Corley
originator
position: Researcher
Pucketty Farm Cottage
Faringdon
SN7 8JP
Oxfordshire
GB
email: martin.corley@btinternet.com
userId: http://orcid.org/0000-0003-4240-8007

Sónia Ferreira
originator
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: hiporame@gmail.com
userId: http://orcid.org/0000-0002-6884-3966

Vanessa Alves Mata
originator
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: vanessamata@cibio.up.pt
userId: http://orcid.org/0000-0003-3005-9030

Pedro Sousa
originator
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: psousa@cibio.up.pt
userId: http://orcid.org/0000-0002-5859-9656

Pedro Beja
originator
position: Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: pbeja@cibio.up.pt
userId: http://orcid.org/0000-0001-8164-0760

Martin Corley
metadata author
position: Researcher
Pucketty Farm Cottage
Faringdon
SN7 8JP
Oxfordshire
GB
email: martin.corley@btinternet.com
userId: http://orcid.org/0000-0003-4240-8007

Sónia Ferreira
metadata author
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: hiporame@gmail.com
userId: http://orcid.org/0000-0002-6884-3966

Vanessa Alves Mata
metadata author
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: vanessamata@cibio.up.pt
userId: http://orcid.org/0000-0003-3005-9030

Pedro Sousa
metadata author
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: psousa@cibio.up.pt
userId: http://orcid.org/0000-0002-5859-9656

Pedro Beja
metadata author
position: Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: pbeja@cibio.up.pt
userId: http://orcid.org/0000-0001-8164-0760

Pedro Beja
administrative point of contact
position: Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: pbeja@cibio.up.pt
userId: http://orcid.org/0000-0001-8164-0760

Sónia Ferreira
administrative point of contact
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: hiporame@gmail.com
userId: http://orcid.org/0000-0002-6884-3966