Quantitative PCR (qPCR) of Key Macroalgal Non-Indigenous Species in Nova Scotia and New Brunswick Waters

Kira Krumhansl; Claudio DiBacco

doi:10.15468/kyhydn

Quantitative PCR (qPCR) of Key Macroalgal Non-Indigenous Species in Nova Scotia and New Brunswick Waters

Citation

Krumhansl K, DiBacco C (2024). Quantitative PCR (qPCR) of Key Macroalgal Non-Indigenous Species in Nova Scotia and New Brunswick Waters. Version 1.1. Fisheries and Oceans Canada. Sampling event dataset https://doi.org/10.15468/kyhydn accessed via GBIF.org on 2024-12-13.

Description

To support the surveillance of key macroalgae and non-indigenous species in Nova Scotia and New Brunswick, five quantitative PCR (qPCR) assays were designed and tested at 111 sites in 2022-2023 targeting the following non-indigenous macroalgal species: Antithamnion sparsum, Bonnemaisonia hamifera, Codium fragile, Dasysiphonia japonica, Fucus serratus. All assays were developed in 2022 by the Center for Environmental Genomics Applications (CEGA, Newfoundland, Canada) except Antithamnion sparsum, for which an assay was developed in 2023 by the Aquatic Biotechnology Laboratory (ABL) at the Bedford Institute of Oceanography. All amplification was performed by the ABL in 2022-2023. The assay developed for Fucus serratus was later determined to be non-specific, and amplifies both F. serratus and Fucus distichus.

Taxonomic Coverages

Geographic Coverages

Nova Scotia and New Brunswick waters

Bibliographic Citations

Krumhansl K.A., Brooks C.M., Lowen B., O’Brien J., Wong M., DiBacco C. Loss, resilience and recovery of kelp forests in a region of rapid ocean warming. Annals of Botany 2024 Mar 8; 133(1):73-92. Brooks C.M., Krumhansl K.A. 2023. First record of the Asian Antithamnion sparsum Tokida, 1932 (Ceramiales, Rhodophyta) in Nova Scotia, Canada. BioInvasions Records 12(3):745-725. -

Contacts

Kira Krumhansl
originator
position: Aquatic Science Biologist
Fisheries and Oceans Canada
1 Challenger Dr
Dartmouth
B2Y 4A2
Nova Scotia
CA
email: Kira.Krumhansl@dfo-mpo.gc.ca

Claudio DiBacco
originator
position: Research Scientist
Fisheries and Oceans Canada
1 Challenger Dr
Dartmouth
B2Y 4A2
Nova Scotia
CA
email: Claudio.DiBacco@dfo-mpo.gc.ca

Kira Krumhansl
administrative point of contact
position: Aquatic Science Biologist
Fisheries and Oceans Canada
1 Challenger Dr
Dartmouth
B2Y 4A2
Nova Scotia
CA
email: Kira.Krumhansl@dfo-mpo.gc.ca

Claudio DiBacco
administrative point of contact
position: Research Scientist
Fisheries and Oceans Canada
1 Challenger Dr
Dartmouth
B2Y 4A2
Nova Scotia
CA
email: Claudio.DiBacco@dfo-mpo.gc.ca