Phytoplankton community composition at station Svartnes, Balsfjord, Norway in June 2017 and June 2018 (sampled with a GoFlow bottle)

Study Extent

All samples were collected at the same station. In 2017, phytoplankton were collected on two days within 1 week, while in 2018, phytoplankton samples were only collected once. On each day, water samples were collected at 6 depths (0, 10, 20, 50, 90, 120 m) for the phytoplankton community composition.

Sampling

To determine the phytoplankton community composition in June 2017 and June 2018, a 30 L GoFlow bottle was successively deployed to 6 water depths (surface, 10, 20, 50, 90, 120 m). The collected water was brought on board and a subsample of 100 mL was transferred into a dark plastic bottle. The sample was preserved with 1 mL of acid lugol and then the sample was stored dark and cool until laboratory analysis within a few months.

Method steps

1. In the laboratory, subsamples were qualitatively and quantitatively analyzed according to the protocols described by Utermöhl (1958) and modified by Edler (1979). For this purpose, 10–50 mL subsamples were poured into the Utermöhl chambers, settled for 24 h in darkness, after which time the protists were counted under an inverted microscope equipped with phase and interference contrasts (Nikon Eclipse TE-300). Microplankton (>20 µm) was enumerated from the entire chamber surface at 100× magnification. Nanoplanktonic cells (3–20 µm) were counted at 400× magnification by moving the field of view along the length of three transverse transects. For the most numerous taxa, up to 50 specimens were counted, and the number of fields of view was considered individually for each count. Systematic affiliation of the identified taxa was verified against the World Register of Marine Species (WoRMS).

N/A

1. Oligotrichida
   rank: order
2. Leptocylindraceae
   rank: family
3. Gymnodiniaceae
   rank: family
4. Dinophyceae
   rank: class
5. Pleurochloridaceae
   rank: family
6. Codonosigaceae
   rank: family
7. Acanthoecida
   rank: order
8. Naviculales
   rank: order
9. Chromista
   rank: kingdom
10. Gonyaulacales
    rank: order
11. Chromulinales
    rank: order
12. Bacillariales
    rank: order
13. Haptophyta
    rank: phylum
14. Naviculaceae
    rank: family
15. Chrysophyceae
    rank: class
16. Cryptophyceae
    rank: class
17. Thalassiosiraceae
    rank: family
18. Prymnesiaceae
    rank: family
19. Gymnodiniales
    rank: order
20. Protozoa
    rank: kingdom
21. Leegaardiellidae
    rank: family
22. Tovelliales
    rank: order
23. Dinophysaceae
    rank: family
24. Prorodontidae
    rank: family
25. NA
    rank: order
26. Actinomonadaceae
    rank: family
27. Leptocylindrales
    rank: order
28. Litostomatea
    rank: class
29. Acanthoecidae
    rank: family
30. Chaetocerotanae incertae sedis
    rank: order
31. Prymnesiales
    rank: order
32. Peridiniaceae
    rank: family
33. Licmophoraceae
    rank: family
34. Thalassiosirales
    rank: order
35. Choanoflagellatea
    rank: class
36. Prostomatea
    rank: class
37. Strombidiida
    rank: order
38. Cladopyxidaceae
    rank: family
39. Prorodontida
    rank: order
40. Oligotrichea
    rank: class
41. Mesodiniidae
    rank: family
42. Pedinellales
    rank: order
43. Peridiniales
    rank: order
44. Cyclotrichiida
    rank: order
45. Myzozoa
    rank: phylum
46. Gonyaulacaceae
    rank: family
47. Protoperidiniaceae
    rank: family
48. Geminigeraceae
    rank: family
49. Prymnesiophyceae
    rank: class
50. Dictyochophyceae
    rank: class
51. Warnowiaceae
    rank: family
52. Xanthophyceae
    rank: class
53. Bacillariophyta
    rank: phylum
54. Pyrenomonadales
    rank: order
55. Tovelliaceae
    rank: family
56. Bacillariophyceae
    rank: class
57. Ochrophyta
    rank: phylum
58. Cryptophyta
    rank: phylum
59. NA
    rank: class
60. Heterocapsaceae
    rank: family
61. Choanozoa
    rank: phylum
62. Strombidiidae
    rank: family
63. Craspedida
    rank: order
64. Dinophysiales
    rank: order
65. Dinobryaceae
    rank: family
66. Chaetocerotaceae
    rank: family
67. NA
    rank: family
68. Ciliophora
    rank: phylum
69. Bacillariaceae
    rank: family
70. Mischococcales
    rank: order
71. Licmophorales
    rank: order
72. Choreotrichida
    rank: order

Station Svartnes, Balsfjorden, Norway (190 m bottom depth)