New Larix-associated Suillus species (Boletales, Agaricomycetes) from North Asia
Citation
Zvyagina E, Sazanova N (2024). New Larix-associated Suillus species (Boletales, Agaricomycetes) from North Asia. Version 1.4. Yugra State University Biological Collection (YSU BC). Occurrence dataset https://doi.org/10.15468/5w33dg accessed via GBIF.org on 2024-12-04.Description
The dataset share information for 13 specimens examined during the description of the new Suillus species from North Asia.The specimens of the Suillus bresadolae f. flavogriseus (Cazzoli & Consiglio) Klofac, Suillus nueschii var. caerulescens Singer, Suillus gastroflavus Zvyagina, Rebriev, Sazanova & E.F. Malysheva and new species are annotated. Annotations are accompanied by geographic data, images of original labels, fruiting bodies, exsicсates and microstructures.Sampling Description
Study Extent
The dataset contains annotations of specimens used for comparative and phylogenetic study, as well as their images and links to DNA sequences in the GenBankSampling
Collections were made, documented and preserved using the standard methods (Bondartsev, Zinger, 1955, Tulloss, 1999, Halling, 1996)Quality Control
All determinations could be checked online or by the request of specimens from the LE and MAG. DNA sequences are deposited in Genbank.Method steps
- Specimens were photographed in situ, examined for taste and smell, dried at temperatures below 50°C.
- Macroscopic descriptions were based on the study of both fresh and dried materials as well as on photographs.
- Microstructures were observed at ×400 and ×1000 in squash preparations in 5% KOH, Congo Red, and Melzer’s reagent. Up to 30 basidiospores, 10 hymenial cystidia, and 10 terminal elements of the pileipellis per specimen were measured to obtain descriptive statistics.
- Measurements were made in ADFImageCapture calibrated by OMP object-micrometre (LOMO). Dimensions are given as (absolute min) average min–average max (absolute max) of the all examined specimens, Q = average min–average max quotient (length/width ratio).
- PCR ITS products were obtained without DNA extraction using the standard protocol of Thermo Scientific Phire Tissue Direct PCR Master Mix kit.
- For amplification of the ITS region the primers ITS1-F, ITS4-B (Gardes, Bruns, 1993) were used.
- LSU (28S) was amplified with LR0R, LR5 (Vilgalys, Hester, 1990) pair of primers, TEF1 with EF1-983F and EF1-1567R (Rehner, Buckley, 2005).
- Amplified products were sequenced using BigDyeH Terminator 3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, California).
- The sequences were assembled in CodonCode Aligner V.9.0.1 (CodonCode Corporation).
Taxonomic Coverages
13 specimens, 5 species, 1 variety, 1 form
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Suillus gastroflavusrank: species
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Ixocomus flavusrank: species
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Suillus nueschii var. caerulescensrank: variety
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Suillus bresadolae f. flavogriseusrank: form
Geographic Coverages
Specimens originated from Italia (Suillus bresadolae f. flavogriseus (Cazzoli & Consiglio) Klofac MCVE18244 Ex Herb Simonini N1844) and Russian Federation from Altay Mountains (Suillus nueschii var. caerulescens Singer LE4547, LE4554), Irkutsk Region (Suillus sp. LE18056, LE18089, LE18421, LE4766) and Magadan Region (Suillus sp. MAG5111, MAG2642, MAG1307, MAG1308, Suillus gastroflavus Zvyagina, Rebriev, Sazanova & E.F. Malysheva Holotype MAG3480 and Paratype MAG5122/LE312702)
Bibliographic Citations
- Gardes, M., Bruns, T.D. (1993) ITS primers with enhanced specifity for Basidiomycetes: application to identification of mycorrhizae and rusts. Mol. Ecol. 2: 113–118. -
- Vilgalys, R., Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4239–4246. -
- Rehner, S.A., Buckley, E. (2005) A Beauveria phylogeny inferred from nuclear ITS and EF1-α sequences: Evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 97: 84–98. - http://dx.doi.org/10.3852/mycologia.97.1.84
- Halling, R. E. (1996) Recommendations for collecting mushrooms for scientific study. Pp. 135-141. In: Alexiades, M. N. and J. W. Sheldon (eds.), Selected Guidelines for Ethnobotanical Research: A Field Manual. The New York Botanical Garden Press, Bronx. -
- Tulloss, R. (1999) Trial Year Notebook for the Great Smoky Mountains National Park All Taxa Biodiversity Inventory. Epigeous Macromycetes. 75 p. -
- Bondartsev, A.S., Singer R.A. (1950) Guidelines for the collection of higher basidial fungi for their scientific study // Proceedings of the Komarov Botanical Institute Academy of Sciences of the USSR. V 2. ( 6). P. 499–572. -
Contacts
Elena Zvyaginaoriginator
position: researcher
Yugra State University
Chekhova street, 16
Khanty-Mansiysk
628012
Khanty-Mansi Autonomous Okrug-Yugra
RU
email: mycena@yandex.ru
userId: http://orcid.org/0000-0003-2063-4847
Nina Sazanova
originator
position: Herbarium curator of fungi
Institute of Biological Problems of the North of the Far East Branch of the Russian Academy of Sciences
Portovaja Street, 18
Magadan
685000
Magadan Region
email: nsazanova_mag@mail.ru
userId: http://orcid.org/0000-0002-0788-1942
Elena Zvyagina
metadata author
position: researcher
Yugra State University
Chekhova street, 16
Khanty-Mansiysk
628012
Khanty-Mansi Autonomous Okrug-Yugra
RU
email: mycena@yandex.ru
userId: http://orcid.org/0000-0003-2063-4847
Nina Sazanova
metadata author
position: Herbarium curator of fungi
Institute of Biological Problems of the North of the Far East Branch of the Russian Academy of Sciences
Portovaja Street, 18
Magadan
685000
Magadan Region
RU
email: nsazanova_mag@mail.ru
userId: http://orcid.org/0000-0002-0788-1942
Nina Filippova
curator
position: senior researcher
Yugra State University
Stroiteley street, 2
Khanty-Mansiysk
628508
Khanty-Mansi Autonomous Okrug-Yugra
RU
email: filippova.courlee.nina@gmail.com
homepage: https://fungariumysu.org/
userId: http://orcid.org/0000-0002-9506-0991
Elena Zvyagina
administrative point of contact
position: researcher
Yugra State University
Chekhova street, 16
Khanty-Mansiysk
628012
Khanty-Mansi Autonomous Okrug-Yugra
RU
email: mycena@yandex.ru
userId: http://orcid.org/0000-0003-2063-4847