Blind mole rat (Spalacidae) sampling, used for phylogenetic analyses
Citation
Rusin M (2024). Blind mole rat (Spalacidae) sampling, used for phylogenetic analyses. Version 1.2. Ukrainian Nature Conservation Group (NGO). Occurrence dataset https://doi.org/10.15468/hfr4gw accessed via GBIF.org on 2024-12-13.Description
The dataset contains detailed sampling data on the mole rats (genus Spalax and Nannospalax) used for phylogenetic analyses.Sampling Description
Study Extent
The dataset consists of records of small rodents from Spalacidae family made on the territory of Romania, Ukraine and Russia in the period from 2009 to 2019 by author with the help of additional resources.Sampling
41 new original samples of seven Spalacidae were collected in Ukraine, Romania and Russia during 2016-2019 years. Additional samples denote material accessible via GenBank (19 samples). Mole rats were collected in the field using hoe technic, gophinator traps or hand-picked (live captured during dispersal above ground or road mortality). Tissue samples were collected in 96% ethanol. DNA isolation and amplification has been performed in the Zonguldak Bülent Ecevit University (Türkiye) and sequencing outsourced in Macrogen (the Netherlands). One mitochondrial gene (full cytochrome b) and one nuclear gene (first exon of IRBP) were obtained for most of the samples. All original nucleotide data uploaded to NCBI GenBank.Quality Control
All species identified in the field, and with genetic markers.Method steps
- Surveys for mole rats: detailed results of surveys available at https://www.gbif.org/dataset/5c216d11-fe9a-4953-b9e4-1feb0b8bf788; https://www.gbif.org/dataset/3cc90eba-6de4-4d39-8f9a-8e1770120f10; https://www.gbif.org/dataset/599f4843-f07a-4369-8c53-ae4fb561f5e1.
- Animal trapping.
- Sample storage in ethanol.
- DNA isolation.
- DNA amplification.
- DNA sequencing.
- Uploading original nucleotide sequences to NCBI GenBank.
- Preparing a dataset of samples according to Darwin Core standards.
Taxonomic Coverages
The dataset consists of rodents from genera Spalax and Nannospalax.
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Animaliarank: kingdom
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Chordatarank: phylum
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Mammaliarank: class
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Rodentiarank: order
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Spalacidaerank: family
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Spalaxrank: genus
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Nannospalaxrank: genus
Geographic Coverages
The dataset consists of records from Romania, Ukraine and Russia.
Bibliographic Citations
- Rusin, M., Çetintaş, O., Ghazali, M., Sándor, A. D., & Yanchukov, A. (2023). Underworld: Evolution of blind mole rats in Eastern Europe. Preprint. -
- Németh A, Homonnay ZG, Krízsik V, et al (2013) Old views and new insights: taxonomic revision of the Bukovina blind mole rat, Spalax graecus (Rodentia: Spalacinae). Zoological Journal of the Linnean Society 169:903–914. -
- Chişamera G, Bužan EV, Sahlean T, et al (2014) Bukovina blind mole rat Spalax graecus revisited: phylogenetics, morphology, taxonomy, habitat associations and conservation. Mammal Review 44:19–29. -
- Stanhope MJ, Czelusniak J, Si JS, et al (1992) A molecular perspective on mammalian evolution from the gene encoding interphotoreceptor retinoid binding protein, with convincing evidence for bat monophyly. Mol Phylogenet Evol 1:148–160. - https://doi.org/10.1016/1055-7903(92)90026-d
- Meredith RW, Janečka JE, Gatesy J, et al (2011) Impacts of the Cretaceous Terrestrial Revolution and KPg extinction on mammal diversification. Science 334:521–524. - https://doi.org/10.1126/science.1211028
- Fang X, Nevo E, Han L, et al (2014) Genome-wide adaptive complexes to underground stresses in blind mole rats Spalax. Nat Commun 5:3966. - https://doi.org/10.1038/ncomms4966
- Steppan SJ, Schenk JJ (2017) Muroid rodent phylogenetics: 900-species tree reveals increasing diversification rates. PLoS One 12:e0183070. - https://doi.org/10.1371/journal.pone.0183070
- Spradling TA, Hafner MS, Demastes JW (2001) Differences in Rate of Cytochrome-b Evolution Among Species of Rodents. J Mammal 82:65–80. - https://doi.org/10.1644/1545-1542(2001)082<0065:DIROCB>2.0.CO;2
Contacts
Mikhail Rusinoriginator
position: researcher
Kyiv Zoo
UA
email: ellobius.talpinus@gmail.com
userId: https://orcid.org/0000-0003-4349-4795
Mikhail Rusin
metadata author
position: researcher
Kyiv Zoo
UA
email: ellobius.talpinus@gmail.com
userId: https://orcid.org/0000-0003-4349-4795
Oleksii Marushchak
custodian steward
position: junior researcher
I. I. Schmalhausen Institute of Zoology NAS of Ukraine
Vul. B. Khmelnytskogo, 15
Kyiv
01030
UA
Telephone: +380964882670
email: ecopelobates@gmail.com
userId: https://orcid.org/0000-0001-9380-5593
Mikhail Rusin
administrative point of contact
position: researcher
Kyiv Zoo
UA
email: ellobius.talpinus@gmail.com
userId: https://orcid.org/0000-0003-4349-4795