Blind mole rat (Spalacidae) sampling, used for phylogenetic analyses

Mikhail Rusin

doi:10.15468/hfr4gw

Blind mole rat (Spalacidae) sampling, used for phylogenetic analyses

Citation

Rusin M (2024). Blind mole rat (Spalacidae) sampling, used for phylogenetic analyses. Version 1.2. Ukrainian Nature Conservation Group (NGO). Occurrence dataset https://doi.org/10.15468/hfr4gw accessed via GBIF.org on 2024-12-11.

Description

The dataset contains detailed sampling data on the mole rats (genus Spalax and Nannospalax) used for phylogenetic analyses.

Sampling Description

Study Extent

The dataset consists of records of small rodents from Spalacidae family made on the territory of Romania, Ukraine and Russia in the period from 2009 to 2019 by author with the help of additional resources.

Sampling

41 new original samples of seven Spalacidae were collected in Ukraine, Romania and Russia during 2016-2019 years. Additional samples denote material accessible via GenBank (19 samples). Mole rats were collected in the field using hoe technic, gophinator traps or hand-picked (live captured during dispersal above ground or road mortality). Tissue samples were collected in 96% ethanol. DNA isolation and amplification has been performed in the Zonguldak Bülent Ecevit University (Türkiye) and sequencing outsourced in Macrogen (the Netherlands). One mitochondrial gene (full cytochrome b) and one nuclear gene (first exon of IRBP) were obtained for most of the samples. All original nucleotide data uploaded to NCBI GenBank.

Quality Control

All species identified in the field, and with genetic markers.

Method steps

Surveys for mole rats: detailed results of surveys available at https://www.gbif.org/dataset/5c216d11-fe9a-4953-b9e4-1feb0b8bf788; https://www.gbif.org/dataset/3cc90eba-6de4-4d39-8f9a-8e1770120f10; https://www.gbif.org/dataset/599f4843-f07a-4369-8c53-ae4fb561f5e1.
Animal trapping.
Sample storage in ethanol.
DNA isolation.
DNA amplification.
DNA sequencing.
Uploading original nucleotide sequences to NCBI GenBank.
Preparing a dataset of samples according to Darwin Core standards.

Taxonomic Coverages

The dataset consists of rodents from genera Spalax and Nannospalax.

Animalia
rank: kingdom
Chordata
rank: phylum
Mammalia
rank: class
Rodentia
rank: order
Spalacidae
rank: family
Spalax
rank: genus
Nannospalax
rank: genus

Geographic Coverages

The dataset consists of records from Romania, Ukraine and Russia.

Bibliographic Citations

Rusin, M., Çetintaş, O., Ghazali, M., Sándor, A. D., & Yanchukov, A. (2023). Underworld: Evolution of blind mole rats in Eastern Europe. Preprint. -
Németh A, Homonnay ZG, Krízsik V, et al (2013) Old views and new insights: taxonomic revision of the Bukovina blind mole rat, Spalax graecus (Rodentia: Spalacinae). Zoological Journal of the Linnean Society 169:903–914. -
Chişamera G, Bužan EV, Sahlean T, et al (2014) Bukovina blind mole rat Spalax graecus revisited: phylogenetics, morphology, taxonomy, habitat associations and conservation. Mammal Review 44:19–29. -
Stanhope MJ, Czelusniak J, Si JS, et al (1992) A molecular perspective on mammalian evolution from the gene encoding interphotoreceptor retinoid binding protein, with convincing evidence for bat monophyly. Mol Phylogenet Evol 1:148–160. - https://doi.org/10.1016/1055-7903(92)90026-d
Meredith RW, Janečka JE, Gatesy J, et al (2011) Impacts of the Cretaceous Terrestrial Revolution and KPg extinction on mammal diversification. Science 334:521–524. - https://doi.org/10.1126/science.1211028
Fang X, Nevo E, Han L, et al (2014) Genome-wide adaptive complexes to underground stresses in blind mole rats Spalax. Nat Commun 5:3966. - https://doi.org/10.1038/ncomms4966
Steppan SJ, Schenk JJ (2017) Muroid rodent phylogenetics: 900-species tree reveals increasing diversification rates. PLoS One 12:e0183070. - https://doi.org/10.1371/journal.pone.0183070
Spradling TA, Hafner MS, Demastes JW (2001) Differences in Rate of Cytochrome-b Evolution Among Species of Rodents. J Mammal 82:65–80. - https://doi.org/10.1644/1545-1542(2001)082<0065:DIROCB>2.0.CO;2

Contacts

Mikhail Rusin
originator
position: researcher
Kyiv Zoo
UA
email: ellobius.talpinus@gmail.com
userId: https://orcid.org/0000-0003-4349-4795

Mikhail Rusin
metadata author
position: researcher
Kyiv Zoo
UA
email: ellobius.talpinus@gmail.com
userId: https://orcid.org/0000-0003-4349-4795

Oleksii Marushchak
custodian steward
position: junior researcher
I. I. Schmalhausen Institute of Zoology NAS of Ukraine
Vul. B. Khmelnytskogo, 15
Kyiv
01030
UA
Telephone: +380964882670
email: ecopelobates@gmail.com
userId: https://orcid.org/0000-0001-9380-5593

Mikhail Rusin
administrative point of contact
position: researcher
Kyiv Zoo
UA
email: ellobius.talpinus@gmail.com
userId: https://orcid.org/0000-0003-4349-4795