Antarctic Peninsula Bacterioplankton 16S rRNA gene surveys and metagenomes from Winter 2002 and Summer 2006.

Alison Murray

doi:10.1098/rstb.2006.1944

Antarctic Peninsula Bacterioplankton 16S rRNA gene surveys and metagenomes from Winter 2002 and Summer 2006.

Citation

Murray A (2019). Antarctic Peninsula Bacterioplankton 16S rRNA gene surveys and metagenomes from Winter 2002 and Summer 2006.. Version 7.1. SCAR - Microbial Antarctic Resource System. Metadata dataset https://doi.org/10.1098/rstb.2006.1944 accessed via GBIF.org on 2023-06-10.

Description

Antarctic surface oceans are well-studied during summer when irradiance levels are high, sea ice is melting and primary productivity is at a maximum. Coincident with this timing, the bacterioplankton respond with significant increases in secondary productivity. Little is known about bacterioplankton in winter when darkness and sea-ice cover inhibit photoautotrophic primary production. We report here an environmental genomic and small subunit ribosomal RNA (SSU rRNA) analysis of winter and summer Antarctic Peninsula coastal seawater bacterioplankton. Intense inter-seasonal differences were reflected through shifts in community composition and functional capacities encoded in winter and summer environmental genomes with significantly higher phylogenetic and functional diversity in winter. In general, inferred metabolisms of summer bacterioplankton were characterized by chemoheterotrophy, photoheterotrophy and aerobic anoxygenic photosynthesis while the winter community included the capacity for bacterial and archaeal chemolithoautotrophy. Chemolithoautotrophic pathways were dominant in winter and were similar to those recently reported in global‘dark ocean’ mesopelagic waters. If chemolithoautotrophy is widespread in the Southern Ocean in winter, this process may be a previously unaccounted carbon sink and may help account for the unexplained anomalies in surface inorganic nitrogen content.

Sampling Description

Study Extent

See Geographic coverage

Sampling

Seawater was collected by submersible pump and filtered at the Station, see Grzymski et al. 2012 for details.

Quality Control

Sanger sequence data was automatically assembled and chimera checked; metagenome sequence data was automatically annotated at the Joint Genome Institute (see Grzymski et al. 2012).

Method steps

A MICROBIAL_SEQUENCE_SET Description file describing 9 data sets was uploaded to the IPT. 9 MIMARKS data files were uploaded to the IPT.

Taxonomic Coverages

Plankton surveys of community structure were conducted of those organisms passing through a 1.6 micron glass fiber filter.

Archaea
rank: domain
Bacteria
rank: domain

Geographic Coverages

Samples were collected in the nearshore region of Anvers Island, near Palmer Station.

Bibliographic Citations

Grzymski, JJ, CS Riesenfeld , TJ Williams, AM Dussaq, H Ducklow, M Erickson, R Cavicchioli, & AE Murray. 2012. A metagenomic assessment of winter and summer bacterioplankton from Antarctic Peninsula coastal surface waters. ISME Journal. - DOI:10.1038/ismej.2012.31
Ghiglione, JF, and AE Murray. 2012. Pronounced summer to winter differences and higher wintertime richness in coastal Antarctic marine bacterioplankton. Environ. Microbiol. 14(3): 617-629. - DOI:10.1111/j.1462-2920.2011.02601.x
Williams, TJ, E Long, F Evans, MZ DeMaere, FM Lauro, MJ Raftery, H Ducklow, JJ Grzymski, AE Murray, R Cavicchioli. 2012. A metaproteomic assessment of summer and winter bacterioplankton from Antarctic Peninsula coastal surface waters. ISME Journal 6:1883-1900. - doi: 10.1038/ismej.2012.28

Contacts

Alison Murray
originator
position: Associate Research Professor
DRI
2215 Raggio Parkway
Reno
89512
NV
US
Telephone: 775 673 7361
email: Alison.Murray@dri.edu
homepage: http://www.dri.edu/alison-murray

Alison Murray
metadata author
position: Associate Research Professor
DRI
2215 Raggio Parkway
Reno
89512
NV
US
Telephone: 775 673 7361
email: Alison.Murray@dri.edu
homepage: http://www.dri.edu/alison-murray

Alison Murray
principal investigator
position: Associate Research Professor
DRI
2215 Raggio Parkway
Reno
89512
NV
US
Telephone: 775 673 7361
email: Alison.Murray@dri.edu
homepage: http://www.dri.edu/alison-murray

Joseph Grzymski
principal investigator
position: Assistant Research Professor
DRI
2215 Raggio Parkway
Reno
89512
NV
US
Telephone: 775 673 7478
email: Joe.Grzymski@dri.edu
homepage: http://www.dri.edu/joe-grzymski

Alison Murray
administrative point of contact
position: Associate Research Professor
DRI
2215 Raggio Parkway
Reno
89512
NV
US
Telephone: 775 673 7361
email: Alison.Murray@dri.edu
homepage: http://www.dri.edu/alison-murray