ASCOMYCETE FUNGI ISOLATED FROM PELTIGERA CYANOLICHENS OF SOUTHERN CHILE

Study Extent

The samples of Peltigera species was collected in Region of Aysén del General Carlos Ibáñez del Campo and Region of Magallanes y de la Antártica Chilena, Chile. The idendification of the yeast isoleted from the lichens was made betewen may and september of 2022

Sampling

Fungi were isolated from thalli of four lichen species of the genus Peltigera (P. frigida, P. fuscopraetextata, P. antarctica, P. rufescens). The thalli were washed twice with sterile distilled water and subsequently macerated. They were then inoculated onto three culture media (Yeast Malt Agar, Potato Dextrose Agar, and a minimal agar medium without amino acids) supplemented with antibiotics. The cultures were incubated in the dark at 10°C for up to 60 days. Long-term preservation of yeast isolates was achieved at -20°C using cryotubes with sterile 15% glycerol, and a dehydrated gelatin drop method at room temperature with sterile 10% gelatin was used. Taxonomic identification was performed by sequencing the ITS marker and subsequent phylogenetic analyses. For the isolates obtained from P. fuscopraetextata substrate, the same procedure was followed, except that no washing step was performed.

Method steps

1. The thalli were washed twice with sterile distilled water and subsequently macerated.
2. They were then inoculated onto three culture media (Yeast Malt Agar, Potato Dextrose Agar, and a minimal agar medium without amino acids) supplemented with antibiotics.
3. The cultures were incubated in the dark at 10°C for up to 60 days.
4. Long-term preservation of yeast isolates was achieved at -20°C using cryotubes with sterile 15% glycerol, and a dehydrated gelatin drop method at room temperature with sterile 10% gelatin was used.
5. Taxonomic identification was performed by sequencing the ITS marker and subsequent phylogenetic analyses.

The present data set reports the taxonomic identification of ascomycete fungi isolated from Peltigera lichen identified at the family, genus and species levels.

1. Fungi
   rank: kingdom
2. Ascomycota
   rank: phylum
3. Arthoniomycetes
   rank: class
4. Dothideomycetes
   rank: class
5. Eurotiomycetes
   rank: class
6. Leotiomycetes
   rank: class
7. Orbiliomycetes
   rank: class
8. Sordariomycetes
   rank: class
9. Chaetothyriales
   rank: order
10. Cladosporiales
    rank: order
11. Coniochaetales
    rank: order
12. Dothideales
    rank: order
13. Eurotiales
    rank: order
14. Hypocreales
    rank: order
15. Leotiomycetes incertae sedis
    rank: order
16. Lichenostigmatales
    rank: order
17. Mycosphaerellales
    rank: order
18. Orbiliales
    rank: order
19. Pleosporales
    rank: order
20. Xylariales
    rank: order
21. Aspergillaceae
    rank: family
22. Barrmaeliaceae
    rank: family
23. Cladosporiaceae
    rank: family
24. Coniochaetaceae
    rank: family
25. Didymellaceae
    rank: family
26. Helotiales incertae sedis
    rank: family
27. Herpotrichiellaceae
    rank: family
28. Nectriaceae
    rank: family
29. Orbiliaceae
    rank: family
30. Phaeococcomycetaceae
    rank: family
31. Pseudeurotiaceae
    rank: family
32. Saccotheciaceae
    rank: family
33. Teratosphaeriaceae
    rank: family
34. Aureobasidium
    rank: genus
35. Calophoma
    rank: genus
36. Cladosporium
    rank: genus
37. Coniochaeta
    rank: genus
38. Constantinomyces
    rank: genus
39. Didymella
    rank: genus
40. Exophiala
    rank: genus
41. Fusarium
    rank: genus
42. Humicolopsis
    rank: genus
43. Lapidomyces
    rank: genus
44. Neosorocybe
    rank: genus
45. Penicillium
    rank: genus
46. Phaeococcomyces
    rank: genus
47. Pseudogymnoascus
    rank: genus
48. Rachicladosporium
    rank: genus
49. Retiarius
    rank: genus
50. Aureobasidium pullulans
    rank: species
51. Calophoma cf. clematidis rectae
    rank: species
52. Constantinomyces aff. nebulosus
    rank: species
53. Didymella cf. macrostoma
    rank: species
54. Humicolopsis aff cephalosporioides
    rank: species
55. Penicillium aff. poederi
    rank: species
56. Pseudogymnoascus aff. roseus
    rank: species

Region of Aysén del General Carlos Ibáñez del Campo, Chile Region of Magallanes y de la Antártica Chilena, Chile